两种不同胚胎装载技术对体外受精-胚胎移植及冻融胚胎移植临床结局的影响

目的探讨两种不同胚胎装载技术对体外受精-胚胎移植(IVF-ET)和冻融胚胎移植(FET)临床妊娠率及胚胎种植率的影响。方法回顾性分析了543个新鲜胚胎移植周期及658个FET周期。根据移植时胚胎装载方法分为A组(含胚培养液体积周围空气体积)和B组(含胚培养液体积周围空气体积)。比较两种胚胎装载方法对妊娠结局的影响。结果新鲜周期中,B组的临床妊娠率(64.52%)显著高于A组(54.62%)(P0.05),种植率组间虽无统计学差异(P0.05),但B组明显高于A组。FET周期中卵裂胚移植B组的临床妊娠率(49.43%)明显高于A组(38.24%),但没有统计学差异(P0.05),B组(34.62%)的种植率显著高于A组(23.61%)(P0.05)。囊胚移植两组的妊娠率和种植率均没有统计学差异(P0.05),但B组比A组有增高的趋势。结论胚胎移植时含胚培养液体积周围空气体积会显著提高IVF-ET和FET的临床妊娠率和种植率。

Impact of two different embryo loading techniques on pregnancy outcome in in vitro fertilization and frozen-thawed embryo transfer cycles

Objective To evaluate the impact of two different embryo loading techniques on pregancy rates and implantation rate in in vitro fertilization and embryo transfer (IVF-ET) cycles and frozen-thawed embryo transfer (FET) cycles.Methods We retrospectively analyzed 543 fresh ET cycles and 658 FET cycles.The patients were placed in group A and group B,group A:the volume of medium containing embryos＞air bubble surrounding it;group B:the volume of medium containing embryos＜air bubble surrounding it.The pregnancy outcome was compared between the two groups.Results In fresh ET cycles,the pregnancy rate in group B (64.52％) was significantly higher than that in group A (54.62％)(P＜0.05).There was no significant difference of implantation rate m groups A and B,but it was higher in group B than in group A obviously.In FET cycles,the cleavage embryo pregnancy in group B was higher than that m group A obviously,but there was no significant difference (P＞0.05).The implantation rate was higher in group B (39.51％) than m group A (26.81％),the difference was significant (P＜0.05).There were no significant differences m both pregnancy rate and implantation rate of blastocyst,but it was higher in group B than in group A.Conclusion The volume of medium containing embryos ＜ air bubble surrounding it can significantly improve the pregnancy rate and imputation rate in fresh ET cycles and FET cycles.