| 以LongSAGE方法寻找不同菌相生长白念珠菌细胞差异表达基因 |
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| 引用本文： | 周村建,郝飞,邓永键,王鲁,钟白玉,李永平.以LongSAGE方法寻找不同菌相生长白念珠菌细胞差异表达基因[J].中华微生物学和免疫学杂志,2006,26(6):564-566. |
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| 作者姓名： | 周村建 郝飞 邓永键 王鲁 钟白玉 李永平 |
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| 作者单位： | 400038,重庆,第三军医大学西南医院皮肤科 |
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| 基金项目： | 国家自然科学基金资助项目(30200013) |
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| 摘 要： | 目的 以长片段基因表达系列分析技术（LongSAGE）寻找酵母相和菌丝相生长的白念珠菌细胞差异表达基因。方法 采用LongSAGE方法，分别构建白念珠菌酵母相和菌丝相细胞Long—SAGE标签文库，比较文库获得不同菌相生长白念珠菌细胞差异表达基因。结果 成功构建了白念珠菌酵母相和菌丝相细胞的LongSAGE标签文库，比较文库获得了白念珠菌酵母相和菌丝相细胞间差异表达的单基因匹配标签。结论 用LongSAGE方法较完整地获得了白念珠菌酵母相和菌丝相细胞基因表达谱及其丰度的量化信息，文库间比较可获得不同菌相生长白念珠菌差异表达基因。
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| 关 键 词： | 白念珠菌 二态性 长片段基因表达系列分析 |
| 修稿时间： | 2005年7月11日 |
Long serial analysis of gene expression of different phases of Candida albicans |
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| ZHOU Cun-jian,HAO Fei,DENG Yong-jian,WANG Lu,ZHONG Bai-yu,LI Yong-ping.Long serial analysis of gene expression of different phases of Candida albicans[J].Chinese Journal of Microbiology and Immunology,2006,26(6):564-566. |
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| Authors: | ZHOU Cun-jian HAO Fei DENG Yong-jian WANG Lu ZHONG Bai-yu LI Yong-ping |
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| Abstract: | Objective To identify the different expressed genes between yeast and hyphal phases of Candida albicans. Methods Two LongSACE libraries were established with long serial analysis of gene expression (LongSAGE) and compared with each other to obtain the different expressed tags within single matching genes. Results Two LongSACE libraries were established successfully. The different expressed tags in yeast and hyphal phases of Candida albicans were obtained separately through libraries comparing. Condusian LongSAGE technology can be used not only in understanding the quantity information of gene expression, but also in finding different expressed genes. |
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| Keywords: | Candida albicans Dimorphism Long serial analysis of gene expression |
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