原位杂交法检测SNCG在乳腺组织中的表达

目的:检测乳腺癌相关基因SNCG在乳腺组织不同病变的阳性表达率,分析其表达规律,探讨其在乳腺癌发生、发展中的作用。方法:用原位杂交方法,以地高辛标记的SNCG反义mRNA作探针,检测167例乳腺不同病变组织中SNCG基因mRNA的表达情况,并进行统计学分析。结果:SNCG的阳性表达率在乳腺增生症为76.0%(38/50),纤维腺瘤为20.0%(3/15),非典型性增生为92.3%(12/13),乳腺癌组织为95.5%(85/89),并且表达强度不同。有淋巴结转移的乳腺癌中强阳性表达率为47.7%(21/44)。而乳腺增生症组中无强阳性表达者。SNCG在不同乳腺病变组织中的表达有统计学意义(P<0.001)。结论:SNCG作为一个新发现的乳腺癌相关基因,在乳腺不同病变中表达情况不同,随病变程度加重,表达率明显增高。有转移者表达强度明显增强。因而SNCG可作为判断乳腺癌恶性潜能的指标。

Detecting Expression of SNCG in benign and malignant breast lesions using mRNA in situ hybridization

Objective:To investigate the expre ss ion of SNCG in benign and malignant breast lesions,and its function in growth and progression of breast cancer.Methods :The ex-pression of SNCG gene in16 7breast tissue samples was detected by in situ hybridization,and studied by st atistical analysis.Results:The positive expression of SNCG was76%(38/5 0)in hy-perpla sia,92.3%(12/13)in atypical hyperplasia and95. 5%(85/89)in breast cancer.The intensity of positive expression was dif ferent.Stronger signal was detected in47.3%cases of advanced in-filtrated breast cancer.However none was detected in hyperplasia(P<0.001).Concl usion:As a novel identified breast cancer related gene,the expression of SNCG was dissimilar in different breast lesions.Its expression rate and strength w ere positively correlated with the proliferative ac-tivity in breast tissue.It is suggested that SNCG might be used as a reference to estimate the malignant potential.