青蒿CQ-189毒副作用研究

目的:以人胚胎神经干细胞和人胚肺成纤维细胞为对象,观察青蒿CQ-189对其体外增殖的影响,并对昆明小鼠主要脏器毒副作用和半数致死量进行观察,旨在探讨青蒿CQ-189的毒副作用.方法:采用MTT法检测青蒿CQ-189对人胚肺成纤维细胞增殖的影响,台盼兰拒染法检测人神经干细胞存活数量.采用小鼠尾静脉给药物法检测半数致死量,组织形态学观察其对小鼠主要器官的毒性作用.结果:青蒿CQ-189在对白血病细胞有较强的抑制作用的有效用药剂量范围内(3.125～12.5 mg·L~(-1))对人胚胎神经干细胞和人胚肺成纤维细胞的毒副作用较低,其对小鼠的半致死剂量为550 mg·kg~(-1),并且在较高浓度作用下对小鼠主要脏器无明显损伤.结论:青蒿CQ-189的毒副作用较低,是很有开发应用前景的抗肿瘤大然成分.

Toxicity and side effects of artemisiae annuae CQ-189

Objective : To observe the effects of artemisiae annuae CQ-189(AACQ-189) on proliferation of hNSC and HELF in vitro, and the main organ toxicity and the median lethal dose(LD₅₀) of kunming mouse in vivo. The purpose is to approach that the toxicity and side effects of AACQ-189. Method : Using techniques of the colorimetric 5-diphenyl tetrazolium bromide(MTT) to detect the effects of AACQ-189 on proliferation of hNSC, and to detect the number of HELF survival by using techniques of trypan blue exclusion. To detect LD₅₀ by tail vein injection in kunming mouse and using histomorphology method to observe the mouse main organ damage by AACQ-189. Result : AACQ-189 has low poisonous function on hNSC and HELF that our experimental concentration(3.125-12.5 mg·L^-1) has already achieve an effective dose to inhibit the proliferation of Leukemia cells obviously. LD₅₀ concentration of kunming mouse is 550 mg·kg^-1. Moreover, AACQ-189 has little effect to main organs at higher concentration. Conclusion : AACQ-189 has low poisonous function, which is a natural anti-tumor drug and has a promising prospect for potential application. However we should do more research on its mechanism.