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| 人胚胎骨髓间充质干细胞在大鼠体内分化为肝细胞的研究 | |
| 引用本文： | 陈焱,梁平,陈耀凯,王宇明,冉崇福,陈理国. 人胚胎骨髓间充质干细胞在大鼠体内分化为肝细胞的研究[J]. 西南国防医药, 2005, 15(1): 4-6 |
| 作者姓名： | 陈焱 梁平 陈耀凯 王宇明 冉崇福 陈理国 |
| 作者单位： | 1. 第三军医大学新桥医院肝胆外科,重庆,400038 2. 第三军医大学西南医院感染科 3. 解放军第452医院 |
| 基金项目： | 国家自然科学基金资助(30370391)。 |
| 摘 要： | 目的：观察人胚胎骨髓间充质干细胞(MSC)在受体大鼠体内的分化演变，研究人胚胎骨髓间充质干细胞在活体大鼠体内转化为人肝细胞的可行性。方法：将人胚胎MSC移植到肝损伤合并自身肝细胞再生抑制大鼠的脾脏。术后分时段取脾脏进行免疫组化染色，了解人白蛋白(ALB)、人角蛋白(CK8)、人CK18、人CK19的表达情况。结果：人胚胎MSC移植后10d大鼠脾脏的人CK8、人CK18、人CK19免疫组化染色可见阳性细胞；移植后15d大鼠脾脏组织内人ALB免疫组化染色可见阳性细胞。结论：人胚胎MSC能够在自身肝细胞再生抑制的肝损伤大鼠脾脏内分化为肝细胞。 |
| 关 键 词： | 骨髓间充质干细胞 分化 细胞移植 |
| 文章编号： | 1004-0188(2005)01-0004-03 |
| 修稿时间： | 2004-12-22 |
Study on differentiation of human embryonic mesenchymal stem cells into hepatocytes in rats |
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| CHEN Yan,LIANG Ping,CHEN Yao - kai,WANG Yu - ming,RAN Cong - fu,CHEN Li - guo :. Study on differentiation of human embryonic mesenchymal stem cells into hepatocytes in rats[J]. Medical Journal of National Defending forces in Southwest China, 2005, 15(1): 4-6 | |
| Authors: | CHEN Yan LIANG Ping CHEN Yao - kai WANG Yu - ming RAN Cong - fu CHEN Li - guo : |
| Affiliation: | CHEN Yan*,LIANG Ping1,CHEN Yao - kai2,WANG Yu - ming2,RAN Cong - fu3,CHEN Li - guo3 1: Department of Infectious Diseases,Southwest Hos pital of Third Military Medical University,Chongqing,400038, 2: Department of Liver and Biliary surgery,Xinqiao Hospital of Third Military Medical University,Chongqing, 3: Hospital 452 of PLA,Chengdu,Sichuan,610021,*: Now working in hospital 452 of PLA. |
| Abstract: | Objective: To study the differentiation of huma n embryonic mesenchymal stem cells(MSC) into hepatocytes in rats. Met hods: Human embryonic MSC were transplanted into the spleens of rats wi th anti - regeneration of hepatocytes and liver injury. After the transplantati on operation, the spleens of these animals were removed at different time based on the experimental schedule, and stained by immunohistochemistry staining metho d. Then the expression of human albumin(ALB), cytokeratin 8(CK8), CK18 and CK19 antigens were detected. Results: The expression of human CK8 , CK18 and CK19 antigens were found in the animals' spleens after ten days of MS C transplantation, and the expression of human ALB antigen was also found in the spleens after fifteen days. Conclusion: Human embryonic MSC can differentiate into hepatocytes in spleens of rats with inhibition of hepato cyte regeneration and liver injury. |
| Keywords: | mesenchymal stem cell differentiation cell tr ansplantation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |