生髓健骨胶囊对酒精性骨质疏松大鼠骨密度、骨矿含量影响的研究

目的从基因分子水平探讨酒精性骨质疏松(alcoholic osteoporosis,AOP)大鼠的发病机制,观察生髓健骨胶囊对AOP大鼠骨密度(bone mineral density,BMD)、骨矿含量(bone mineral content,BMC)表达的影响,探讨生髓健骨胶囊对AOP大鼠模型的中药防治作用机理。方法选取成年雄性(清洁级)SD大鼠120只,称体重,随机分为4组,每组各30只,用白酒灌胃法造模,同时分别给予生理盐水、碳酸钙阿法D3、生髓健骨胶囊灌胃给药。于造模8、12、16周末取材,检测股骨上端BMD、BMC指标。结果检测造模干预8、12、16周后BMD、BMC指标变化,模型组BMD、BMC与正常组比较明显降低,且差异有统计学意义(P0.01),结果表明饮酒大鼠确实存在骨量减少,BMD、BMC降低;中药干预组BMD、BMC与模型组相比显著升高(P0.01);中药干预组BMD、BMC与西药对照组相比,明显升高(P0.05)。结论通过观察生髓健骨胶囊对AOP大鼠的实验指标,证明了生髓健骨胶囊能够提高AOP大鼠骨密度,增加骨矿含量,抑制矿物质丢失,改善大鼠的骨代谢。

Effect of the generating marrow and strong-bone capsule on bone mineral density and bone mineral content in rats with alcoholic osteoporosis

Objective To explore the pathogenesis of alcohol-induced osteoporosis (AOP) in rats from the molecular gene level, to observe the effect of the generating marrow and strong-bone capsule on bone mineral density (BMD) and bone mineral content (BMC) in AOP rats, and to discuss the preventive and therapeutic mechanism of generating marrow and strong-bone capsule in AOP rats. Methods One hundred and twenty adult male SD rats (clean grade) were weighted and randomly divided into 4 groups, with 30 rats in each group. The rats were modelled with Red Star Erguotou liquor gavage method. At the same time, normal saline, calcium carbonate alfa-D3 and generating marrow and strong-bone capsule were gavaged, respectively. On 8, 12, and 16 weeks, BMD and BMC of the upper femur were detected. Results Software SPSS 22.0 was used for statistical analysis (P<0.05) and t test was performed. The changes of BMD and BMC at 8, 12 and 16 weeks after modeling intervention were detected. BMD and BMC in model group and normal group decreased significantly, and there was significant difference (P<0.01). The results showed that there was a decrease in bone mass and BMD and BMC in rats taking alcohol. Compared with model group, BMD and BMC in Chinese medicine intervention group increased significantly (P<0.01). Compared with western medicine control group, BMD and BMC increased significantly (P<0.05). Conclusion By observing the experimental index of the generating marrow and strong-bone capsule on AOP rats, it is proved that the generating marrow and strong-bone capsule increases BMD and BMC, inhibits mineral loss, and improves bone metabolism in AOP rats.