| Specific Binding of 17D3 Anti-pZP mAb to Porcine and Human Zona PeHucida but not to Other Tissue Antigens Identified by ABC Immunohistochemistry |
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| 作者姓名： | Min-min YUAN Da-jin LI Chao-jing LI Bai-qing GE Ying ZHU Min-yan WANG |
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| 作者单位： | Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics and Gynecology, ShanghaiMedical College, Fudan University, Shanghai 200011, China |
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| 基金项目： | This work was supported by 985 grants of Fudan University （985B36） |
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| 摘 要： | Objective To characterize the binding effects of 17D3 anti-pZP monoclonal antibody （mAb） on porcine and human ZP,, ovary and other important tissues Methods The 17D3 anti-pZP mAb was produced by immunizing mouse with porcine zona pellucida and hybridoma and monoclonal antibody preparation. An ABC immunohistochemistry was used to evaluate reaction of mAb 17D3pZP to porcine and human ZP antigens as well as important human tissue antigens. Results mAb 17D3pZP specifically bound to porcine and human ZP antigen, but not to other cells of ovaries and other important human tissue antigens. Conclusion 17D3 anti-pZP mAb can recognize porcine and human ZP antigen without cross-reaction with other human tissue antigens including ovary, so it may further help us to abstract and purify corresponding target antigen and settle basis for producing human ZP contraceptive vaccine.
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| 关 键 词： | 17D3抗-pZP单克隆抗体 猪 动物实验 人体实验 带状疮疹 组织抗原 |
| 收稿时间： | 2004-12-11 |
Specific Binding of 17D3 Anti-pZP mAb to Porcine and Human Zona PeHucida but not to Other Tissue Antigens Identified by ABC Immunohistochemistry |
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| Min-min YUAN Da-jin LI Chao-jing LI Bai-qing GE Ying ZHU Min-yan WANG.Specific Binding of 17D3 Anti-pZP mAb to Porcine and Human Zona PeHucida but not to Other Tissue Antigens Identified by ABC Immunohistochemistry[J].Journal of Reproduction and Contraception,2005,16(1):17-22. |
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| Authors: | Min-min;YUAN;Da-jin;LI;Chao-jing;LI;Bai-qing;GE;Ying;ZHU;Min-yan;WANG |
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