| 肺炎支原体感染患儿免疫功能变化及意义 |
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| 引用本文： | 汪燕,王昊,王雪方,孙博.肺炎支原体感染患儿免疫功能变化及意义[J].中国卫生检验杂志,2011(1). |
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| 作者姓名： | 汪燕 王昊 王雪方 孙博 |
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| 作者单位： | 杭州市第一人民医院中心实验室; |
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| 摘 要： | 目的:检测肺炎支原体(mycoplasma pneumoniae,MP)感染患儿细胞免疫和体液免疫功能,研究其变化规律,探讨疾病发生机制。方法:收集支原体肺炎患儿急性期全血和血清以及健康儿童对照标本,采用流式细胞仪检测淋巴细胞表面标记CD3+、CD4+、CD8+、NK、CD19+、CD3+HLA-DR+、CD3+CD25+、CD8+HLA-DR+、CD8+CD38+和调节性T细胞CD4+CD25+表达比例。采用IMMAGE特定蛋白分析仪检测血清标本IgG、IgA、IgM、C3和C4浓度。结果:与正常对照组比较,支原体肺炎患儿组急性期外周血CD3+、CD8+CD38+和CD4+CD25+细胞比例明显下降,B淋巴细胞(CD19+细胞)比例则显著升高,血清中IgG和IgA含量明显下降。结论:支原体肺炎患儿急性期处于免疫失衡状态,T淋巴细胞增殖受到抑制,B淋巴细胞刺激增殖,体液免疫功能仍存在一定失调。
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| 关 键 词： | 肺炎支原体 流式细胞仪 细胞免疫 体液免疫 |
Detection and analysison the immunologic function of children infected with mycoplasma pneumoniae |
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| WANG Yan,WANG Hao,WANG Xue-fang,SUN Bo.Detection and analysison the immunologic function of children infected with mycoplasma pneumoniae[J].Chinses Journal of Health Laboratory Technology,2011(1). |
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| Authors: | WANG Yan WANG Hao WANG Xue-fang SUN Bo |
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| Abstract: | Objective:The cellular immunity and humoral immunity function of children infected with mycoplasma pneumoniae(MP) were detected to investigate the changes,index and the mechanism of this disease.Methods:Peripheral blood and serum samples of healthy children and children infected with MP in acute stage were collected,CD3+,CD4+,CD8+,NK,CD19+,CD3+HLA-DR+,CD3+CD25+,CD8+HLA-DR+,CD8+CD38+ and CD4+CD25+ were detected by Flow Cytometry.The serum concentration of IgG,IgA,IgM,C3 and C4 were detection by IMMAGE.Result... |
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| Keywords: | Mycoplasma Pneumoniae Flow Cytometry Cellular Immunity Humoral Immunity |
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