应用cDNA微阵列技术研究胶质母细胞瘤凋亡相关基因的表达

目的研究胶质母细胞瘤凋亡相关基因的表达。以探讨凋亡相关基因的异常表达在肿瘤的发生、发展过程中的作用。方法采用cDNA微阵列技术,研究胶质母细胞瘤与正常脑组织之间凋亡相关基因表达的差异。采用看家基因对杂交信号进行校正。差异大于1.5倍的基因视为差异有显著性意义,并从中选择6个基因采用RT-PCR进行验证。结果共有28个基因的表达存在差异,其中有17个基因在胶质母细胞瘤表达升高,11个表达下降。RT-PCR结果与基因芯片结果一致。结论众多凋亡相关基因参与了胶质母细胞瘤的发生和发展,其中部分基因如CRAF1、hHR23B等是新发现的与胶质母细胞瘤有关的基因。

Screening the Various Expression of Apoptosis Related Genes between Glioblastoma and Normal Brain Tissue with cDNA Microarray

Objective To Screen the various expression of apoptosis related genes between glioblastomas and normal brain tissue with cDNA microarray. Methods Totally 12 tumor samples and 8 normal brain tissues were used in the experiments. Tumor samples were obtained from the patients who underwent operation in our department, and the control brain tissue were obtained from the patients of epilepsy who underwent lobe-ectomy in our department. The various expression of apoptosis associated genes between glioblastoma and normal brain tissue were screened by means of a cDNA microarray. The hybridized signal was normalized using the signal of a set of house-keeping genes. The gene whose signal change was more than 1.5 times were selected and considered at significantly different levels. Results Totally, 28 differential expression genes were found in the experiments including 17 up-regulation and 11 down-regulation. 6 of them were selected and corroborated using RT-PCR. The results of RT-PCR of the selected genes showed that the direction of expression alteration were identical with the results of cDNA analysis. Conclusion A lot of apoptosis related genes involve in the development of glioblastoma, and CRAF1, hHR23B are the new genes which related to glioblastoma.