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The inhibitory effect of Houttuynia cordata extract on stem cell factor-induced HMC-1 cell migration
Authors:Kim In Sik  Kim Joo-Hwan  Kim Jin Sook  Yun Chi-Young  Kim Dong-Hee  Lee Ji-Sook
Affiliation:Department of Biomedical Laboratory Science, School of Medicine, Eulji University, 143-5 Yeuongdu-dong, Jung-gu, Daejeon 301-832, Republic of Korea. orientree@eulji.ac.kr
Abstract:
Hottuynia cordata Thunb (Saururaceae; HC) is known as a therapeutic drug that has been used in traditional oriental medicine for the treatment of allergy. Mast cells play an important role in a variety of inflammatory diseases, and specifically asthma and atopy. In the present study, we investigated the effect of HC extracts on the migration of the human mast cell line, HMC-1, in response to stem cell factor (SCF). Treatment with HC extracts at a concentration of 10mug/ml for 24h showed no significant decrease in the survival rate of the HMC-1 cells. SCF showed the typical bell-shape curve for the HMC-1 cell chemoattraction with the peak of the curve at the SCF concentration of 100ng/ml. HC-1, which was the whole plant (Houttuynia cordata) extracted with 80% EtOH, and HC-3, which was the residue successively partitioned with EtOAc, both had inhibitory effects on HMC-1 cell movement. After the treatment with 10mug/ml HC-1 extract for 6 and 24h, the chemotactic index (CI) of HMC-1 cells decreased up to 74 and 63%, respectively. HC-3 extract treatment for 6 and 24h lowered the CI to 72 and 44%, respectively. The HC-1 and HC-3 extracts had no inhibitory effect on the mRNA and surface protein expressions of c-kit, SCF receptor. SCF mediated the chemotaxis signaling via NF-kappaB activation, and both extracts inhibited the activation. Therefore, our results indicate that HC-1 and HC-3 extracts decrease the chemotactic ability of HMC-1 cells in response to SCF by inhibiting the NF-kappaB activation, and these substances may be useful for treating mast cell-induced inflammatory diseases.
Keywords:BuOH, butanol   CI, chemotactic index   ERK, extracellular signal-regulated kinase   EtOAc, ethyl acetate   EtOH, ethanol   HC, Hottuynia cordata Thunb   JAK, Janus kinase   JNK, Jun-N-terminal kinase   MCs, mast cells   MAPK, mitogen activated protein kinase   MEK, MAPK kinase   NF-κB, nuclear factor-kappa B   PI3K, phosphoinositol-3 kinase   SCF, stem cell factor
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