内毒素性急性肺损伤大鼠白细胞介素—6含量改变

采用白细胞介素－６（Ｉｎｔｅｒｌｅｕｋｉｎ６，ＩＬ－６）依赖细胞株７ＴＤ１及ＭＴＴ比色法，动态观察了正常及内毒素注射后不同时间大鼠血清和支气管肺泡灌洗液（ｂｒｏｎｃｈｏａｌｏａｌｖｅｏｌａｒｌａｖａｇｅｆｌｕｉｄ，ＢＡＬＦ）中ＩＬ－６含量的变化。同时，还对各组大鼠的肺体指数和ＢＡＬＦ中蛋白质含量进行了测定，结果显示：大鼠注射内毒素后１，３，６，１２ｈ其血清和ＢＡＬＦ中ＩＬ－６含量急剧升高（Ｐ〈０．

Changes of serum and BALF IL-6 level and their effect on the pathogenesis of acute lung injury

Changes of IL-6 contents in serum and bronchoalveolar lavage fluid (BALF) in rats of normal and after endotoxin infusion were measured using the IL-6 dependent cell line 7TD1 and MTT colorimetric assay, and the lung-body index and protein contents in rat's BALF were also measured. The results showed that the serum and BALF IL-6 contents were sighficantly higher (P<0.01) at 1, 3,6, 12h after endotoxin infusion and returned to the baseline at 24h after endotoxin. Moreover, the lung-body index and protein content in Bas were also increased sharply after endotoxin infusion, especially at 3,6h after infusion. There were a positive relationship betWeen the IL-6 content in plasma or BALF and the protein content in BALF after endotokin infusion. The findings indicated that elevated serum and BALF IL-6 level is important in the pathogenesis of acute lung injury.