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| 人参总甙抗四氯化碳损伤大鼠离体肝细胞的作用 | |
| 引用本文： | 吴斌 王崇文. 人参总甙抗四氯化碳损伤大鼠离体肝细胞的作用[J]. 中国病理生理杂志, 1997, 13(6): 681-684 |
| 作者姓名： | 吴斌 王崇文 |
| 作者单位： | 江西医学院第一附属医院消化科!南昌，330006，江西医学院第一附属医院消化科!南昌，330006，江西医学院第一附属医院消化科!南昌，330006，江西医学院第一附属医院消化科!南昌，330006 |
| 摘 要： | 本实验采用大鼠离体肝细胞原代培养２４ｈ并利用ＣＣｌ４造成急性细胞损伤模型，检定人参总甙（ＧＳＳＤ）对肝细胞损伤的影响。结果表明：ＧＳＳＤ可显著降低中毒肝细胞的脂质过氧化物水平。抑制肝细胞脂质过氧化，并降低谷丙转氨酶（ＧＰＴ）和谷草转氨酶（ＧＯＴ）水平，保护脂质膜。ＧＳＳＤ显著促进中毒肝细胞ＲＮＡ和ＤＮＡ的合成，超微结构观察证实ＧＳＳＤ能减轻ＣＣｌ４对肝细胞染色质，线粒体，内质网和核蛋白体的损害，结 |
| 关 键 词： | 人参总甙 四氯化碳 肝损伤 气体中毒 |
Protective effect of ginsenoside against CCl4- induced primary cultured rat hepatocytes injury |
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| Wu Bin,Wang Chong-Wen,Xu Jia-Rui,Zhu Jin-Quan. Protective effect of ginsenoside against CCl4- induced primary cultured rat hepatocytes injury[J]. Chinese Journal of Pathophysiology, 1997, 13(6): 681-684 | |
| Authors: | Wu Bin Wang Chong-Wen Xu Jia-Rui Zhu Jin-Quan |
| Abstract: | The effect of ginsenoside (GSSD) on CCl4- induced primary cultured rat hepalocytes injury were examined. GSSD treatment (300mg. L -1) attenuated CCl4 (10mmol. L -1) - induced damages of primary cultured rat hepatocfytes significantly as indicated by decreaes of GPT and GOT leakage and LPO production. GSSD promoted incorporation of [3H] - UR into RNA and of [3H] -TdR into DNA sigllificantly in the rat hepatocytes. Cytopathological study showed that GSSD attenuated CCl4 damage of heterochromatin, mitochondrion, endoplasimic reticulum and ribosome. These results indicated that by reducing lipid peroxidation and promoting RNA and DNA synthesis, GSSD has notable protective effect against CCl4- induced damage of primary cultured rat hepatocytes. |
| Keywords: | Ginsenoside Liver Carbon tetrachloride poisoning |
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