10种天然黄酮化合物对大鼠心肌细胞抗凋亡作用的定量构效关系研究

目的利用10种天然黄酮化合物对大鼠心肌细胞抗凋亡活性数据，对10种黄酮化合物的2个理化参数（脂水分配系数logP和游离羟基数N）进行定量构效关系研究（QSAR），找出最佳拟合方程——Hansch方程，以指导黄酮类化合物的结构最佳化研究。方法利用过氧化氢诱导心肌细胞损伤模型，用MTT法测定10种天然黄酮化合物的抗凋亡作用，利用统计软件SPSS13．0进行多元线性回归分析。结果得到黄酮化合物抗凋亡作用的Hansch方程。结论通过统计分析，所选定的天然黄酮化合物的Hansch方程具有较好的拟合性（n=10，r=0．807，SE=0．0920，F=6．541，Q=8．77）。

QSAR study of antiapoptosis of 10 natural flavones on rat cardiocyte

Aim To obtain the best fitting equation,Hansch equation between the anti-apoptosis activity of 10 tested natural flavones on rat cardiocyte and two physicochemical properties,logP and number of free hydroxyl group(N),the anti-apoptosis activity data of flavones and physicochemical properties were utilized on quantitative structure-activity relationships(QSAR) study.Then further exploitation of anti-apoptosis flavone derivatives could be guided.Methods The cardiocyte damage model was induced by H2O2.The anti-apoptosis ratio of 10 natural flavones were tested in the MTT assay.The multiple linear regression analyses were carried out with the statistical SPSS(version 13.0).Results The Hansch equation on anti-apoptosis of flavones was obtained.Conclusion The fitting of Hansch equation of natural flavones was satisfactory by statistical analysis(n=10,r=0.807,SE=0.0920,F=6.541,Q=8.77).