新疆维吾尔族妇女宫颈上皮内瘤变及宫颈癌中树突状细胞的检测及意义

目的探讨树突状细胞在新疆维吾尔族妇女宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)及宫颈癌中的检测及意义。方法 将HLA-DR、CD1a、S-100蛋白作为DC特异性标记物,采用免疫组织化学方法对20例慢性宫颈炎、70例CIN(CINⅠ25例、CINⅡ25例、CINⅢ20例)以及40例浸润性宫颈鳞癌组织标本中DC的分布和表达进行检测。结果 S-100、CD1a和HLA-DR阳性表达率依次为浸润性宫颈鳞癌(65.00%,55.00%,62.50%)>CIN(50.00%,44.29%,40.00%)>慢性宫颈炎(30.00%,20.00%,25.00%),差异具有统计学意义(P=0.035,P=0.037,P=0.012),S-100、CD1a和HLA-DR分布于癌组织及癌巢附近的间质中。在宫颈各组织中,CD1a与S-100蛋白表达呈正相关(r=0.330,P=0.000);CD1a与HLA-DR表达也呈正相关(r=0.267,P=0.002);而S-100蛋白与HLA-DR表达没有相关性(r=0.065,P=0.461)。结论人宫颈上皮内瘤变至浸润癌变过程中树突状细胞特异性标记物S-100、HLA-DR、CD1a表达强度发生变化,提示宫颈病变局部的免疫功能发生了改变。

Detection and significance of dendritic cells in Xinjiang Uygur women cervical intraepithelial neoplasia and cervical cancer

Objective To study detection and clinical significance of the dendritic cells in Xinjiang Uygur women cervical intraepithelial neoplasia （ CIN ） and cervical cancer. Methods The expressions of HLA-DR, CDla and S-100 protein （ markers of dendritic cells ） were studied using immunohistoehemical SP method to detect the distribution and expression of dendritic cells in 20 cases of chronic cervicitis, 70 cases of CIN （ C1N 1 25, CIN II 25 , CIN ill 20 ） and 40 cases of invasive cervical squamous ceil carcinoma specimens. Results The positive rates of S-100, HLA-DR and CDla expression were as follows： in invasive cervical squamous cell carcinoma （ 65.00%, 62.50%, 55.00% ） 〉 CIN （ 50.00%, 40.00%, 44.29%） 〉 chronic cervieitis （ 30.00%, 25.00%, 20.00% ） with significant difference statistically （P=0.035, P =0.012, P=0.037 ） and with their products distributed in cancerous tissue and nests nearby stroma. The expression of CDla and S-lO0 protein was positively correlated （ r = 0.330, P=0.000） and the expression of CDla and HLA-DR was positively correlated （ r = 0.267, P =0.002 ） , but the expressions of HLA-DR and S-100 protein were ineorrelated （ r =0.065, P =0.461 ） . Conclusion The aherations of expression intensity of HLADR, CDla and S-100 protein in the process of cervical carcinogenesis indicates the changes of local immunologic function in human palhologieal cervix.