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| 荧光光谱法研究芒柄花素与牛血清白蛋白的相互作用 | |
| 引用本文： | 罗天雪,杜迎翔,汪豪. 荧光光谱法研究芒柄花素与牛血清白蛋白的相互作用[J]. 海峡药学, 2011, 23(5): 245-248 |
| 作者姓名： | 罗天雪 杜迎翔 汪豪 |
| 作者单位： | 1. 中国药科大学分析化学教研室,南京,210009 2. 中国药科大学分析化学教研室,南京,210009;中国药科大学药物质量与安全预警教育部重点实验室,南京,210009;中国药科大学现代中药教育部重点实验室,南京,210009 3. 中国药科大学天然药物化学教研室,南京,210009 |
| 摘 要： | 在模拟动物体生理条件下,用荧光光谱法研究芒柄花素(hq-2)与牛血清白蛋白(BSA)的结合作用.结果 表明,芒柄花素对BSA荧光猝灭属于静态猝灭;并求得结合常数Ka为5.27×104L·mol-1,结合位点数为1.热力学数据表明该药物与牛血清白蛋白的相互作用是一个吉布斯自由能降低的自发过程,且二者之间的主要作用力类型为... |
| 关 键 词： | 芒柄花素 牛血清白蛋白 荧光光谱 相互作用 |
Study on the interaction between fermononetin and bovine serum albumin by fluorescence spectroscopy |
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| LUO Tian-xue,DU Ying-xiang,WANG Hao. Study on the interaction between fermononetin and bovine serum albumin by fluorescence spectroscopy[J]. Strait Pharmaceutical Journal, 2011, 23(5): 245-248 | |
| Authors: | LUO Tian-xue DU Ying-xiang WANG Hao |
| Affiliation: | 1.Department of Analytical Chemistry,China Pharmaceutical University,Nanjing 210009,China;2.Key Laboratory of Drug Quality Control and Pharmacovigilance(Ministry of Education),China Pharmaceutical University,Nanjing 210009,China;3.Key Laboratory of Modern Chinese Medicines(Ministry of Education),China Pharmaceutical University,Nanjing 210009,China;4.Department of Natural Medicinal Chemistry,China Pharmaceutical University,Nanjing 210009,China) |
| Abstract: | To study the interaction of bovine serum albumin(BSA)with fermononetin(hq-2)by fluorescence quenching spectra and synchronous fluorescence spectra.The results indicated that the quenching type between BSA and fermononetin was static quenching.The binding constants Ka were 5.27×104L·mol-1 and the binding sites(n)were 1.The interaction between BSA with fermononetin was driven mainly by Electrostatic interaction.And the synchronous spectrum was used to investigate the conformational changes of BSA.The competitive probes,such as warfarin and ibuprofen(Site Ⅰ and Site Ⅱ probes,respectively),revealed that the binding location of fermononetin to BSA was in the site Ⅱ of the hydrophobic pocket. |
| Keywords: | fermononetin BSA Fluorescence spectroscopy Interaction |
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