| HSP90多肽复合物对树突状细胞CD86、CD11c及IL-12p70表达的影响 |
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| 引用本文： | 赵静,汪小鹏,吴开松,林宇辉. HSP90多肽复合物对树突状细胞CD86、CD11c及IL-12p70表达的影响[J]. 实用预防医学, 2011, 18(4): 588-590 |
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| 作者姓名： | 赵静 汪小鹏 吴开松 林宇辉 |
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| 作者单位： | 武汉大学中南医院呼吸内科,湖北,武汉,430071 |
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| 摘 要： | 目的研究HSP90多肽复合物对树突状细胞（dendritic cells,DC）功能的影响,并探讨HSP90多肽复合物修饰的DC疫苗对肿瘤的抑制作用。方法 HSP90多肽复合物刺激DC前后,ELISA法检测IL-12P70的表达水平,流式细胞仪检测CD86及CD11c的表达。建立C57BL/6小鼠Lewis肺癌模型,观察不同组别DC疫苗对肿瘤生长的抑制作用。结果 DC经HSP90多肽复合物刺激后,CD86及CD11c的表达上调（94.49±1.49及81.87±1.64）%,IL-12p70分泌量（97.11±5.26）pg/ml,均高于肿瘤粗提取物刺激DC组及未刺激空白DC组（P〈0.05）。HSP90多肽复合物修饰的DC疫苗可明显抑制肿瘤生长,抑制率为57.56%,凋亡率为26.35%,均明显高于对照组（P〈0.05）。结论 HSP90多肽复合物能够诱导DC成熟,经其修饰的DC能抑制肿瘤生长,可能与其能诱导肿瘤细胞凋亡有关。
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| 关 键 词： | 肿瘤 热休克蛋白90多肽复合物 树突状细胞 |
Effect of HSP90 Peptide Complex on Expression of CD86,CD11c and IL-12p70 in Dendritic Cells |
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| ZHAO Jing,WANG Xiao-peng,WU Kai-song,et al.. Effect of HSP90 Peptide Complex on Expression of CD86,CD11c and IL-12p70 in Dendritic Cells[J]. Practical Preventive Medicine, 2011, 18(4): 588-590 |
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| Authors: | ZHAO Jing WANG Xiao-peng WU Kai-song et al. |
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| Affiliation: | ZHAO Jing,WANG Xiao-peng,WU Kai-song,et al.(Department of Respiratory,Zhongnan Hospital of Wuhan University,Wuhan 430071,Hubei,China) |
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| Abstract: | Objective To study the effect of HSP90 peptide complex on dendritic cells(DC),and to explore the antitumor effect of dendritic cell vaccine modified by HSP90 peptide complex. Methods IL-12p70 production was detected by ELISA and the expression of CD86 and CD11c was analyzed by flow cytometry before and after DC stimulating by HSP90 peptide complex.C57BL/6 mouse models of Lewis lung cancer were established to observe the inhibition of tumor by different groups of dendritic cell vaccine. Results After DC stim... |
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| Keywords: | Tumor Heat shock protein 90 peptide complex Dendritic cells |
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