器官培养中骨吸收因子的相互作用

为了研究不同浓度脂多糖（ｌｉｐｏｐｏｌｙｓａｃｃｈａｒｉｄｅ，ＬＰＳ）、脂磷壁酸（ｌｉｐｏｔｅｉｃｈｏｉｃａｃｉｄ，ＬＴＡ）、肿瘤坏死因子（ｔｕｍｏｕｒｎｅｃｒｏｓｉｓｆａｃｔｏｒ，ＴＮＦ）及前列腺素Ｅ（ｐｒｏｓｔａｇｌａｎｄｉｎＥ，ＰＧＥ）的骨吸收作用和相互作用，采用４５Ｃａ标记的１９天胚胎鼠长骨干器官培养，用样品道比法定量分析。结果表明，上述因子均能在器官培养水平刺激骨吸收，与对照组相比差异有显著性（Ｐ＜０．０５）。低浓度的ＬＰＳ与ＴＮＦ、ＬＰＳ与ＰＧＥ、ＴＮＦ与ＰＧＥ相互作用，超过各因子单独作用之和（期望值）；ＬＰＳ与ＬＴＡ、ＴＮＦ与ＬＴＡ相互作用，等于期望值；而ＬＴＡ与ＰＧＥ相互作用，小于期望值。研究结果提示，多种骨吸收因子的相互作用在牙周病骨吸收发病机理中有重要意义。

Interactions among stimulators of bone resorption in organ culture

The labeled 45Ca long bone shafts of 19 day fetal rats in organ culture was used in the study. Actions and interactions between various concentrations of LPS, TNF, LTA and PGE were quantitatively analysed by sample channels ratio method. The results indicated that they could all induce bone resorption in organ culture and show significance of differences assessed using student's test (P < 0.05). Interacts between LPS and TNF, LPS and PGE, TNF and PGE were greater than the sum of the individual responses when added together (the expection value), LPS and LTA, TNF and LTA were equal to the expection value, but LTA and PGE were less than the expection value. The result supported that the interactions among these stimulators of bone resorption were important in the pathogenesis of bone loss in periodontal disease.