| 高效液相色谱法测定左旋尤利沙星的含量及有关物质 |
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| 引用本文： | 满凤,安穗伟,梁北梅,彭锋,刘学斌,李发美. 高效液相色谱法测定左旋尤利沙星的含量及有关物质[J]. 中国抗生素杂志, 2010, 35(2) |
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| 作者姓名： | 满凤 安穗伟 梁北梅 彭锋 刘学斌 李发美 |
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| 作者单位： | 1. 沈阳药科大学药学院,沈阳,110016
2. 广州市医药工业研究所,广州,510240 |
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| 摘 要： | 目的 建立测定左旋尤利沙星含量及有关物质的高效液相色谱方法.方法 采用Diamonsil C_(18)柱(250mm×4.6mm,5μm),以乙腈-0.2%三乙胺和0.1%庚烷磺酸钠水溶液(用磷酸调节pH 2.5)(25:75)为流动相,流速为1.0ml/min,检测波长为275nm,柱温为35℃.结果 主峰能与相邻杂质峰达基线分离,左旋尤利沙星的浓度在10～200μg/ml范围内线性关系良好,相关系数为0.9996,最低检测限为0.2ng.结论 该法简便、准确,灵敏度高,专属性强,可以用于左旋尤利沙星的含量及有关物质的测定.
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| 关 键 词： | 左旋尤利沙星 含量测定 有关物质 |
Determination of the content of levoulifloxacin and its related substances by HPLC |
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| Man Feng,An Sui-wei,Liang Bei-mei,Peng Feng,Liu Xue-bin,Li Fa-mei. Determination of the content of levoulifloxacin and its related substances by HPLC[J]. Chinese Journal of Antibiotics, 2010, 35(2) |
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| Authors: | Man Feng An Sui-wei Liang Bei-mei Peng Feng Liu Xue-bin Li Fa-mei |
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| Abstract: | Objective To develop an HPLC method for determination of levoulifloxacin and its related substances.Methods The chromatographic separation of levoulifloxacin was performed on a Diamonsil C_(18) column with a mobile phase consisting of acetonitrile-0.2%triethylamine and 0.1%sodium heptanesulfonic acid at pH 2.5 the column temperature was maintained at 35℃.Results Levoulifloxacin was completely separated from its related substances.The calibration curve of levoulifloxacin was linear in the range of 10-200 μg/ml with the correlation coefficient of 0.9996.The limit of detection was 0.2ng.Conclusions The method is simple,rapid,sensitive and specific,and can be used for determination of levoulifloxacin and its related substances. |
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| Keywords: | HPLC Levoulifloxacin HPLC Determination Related Substances |
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