Modified method of injecting parental lymphocytes to induce a local graft versus host reaction

A new method of injecting parental lymphocytes into the foot of F₁ hybrid mice to induce a local graft versus host reaction, based on the use of the Achilles' tendon as a natural shutter covering the lumen of the wound channel, is suggested. The new method of injection greatly simplifies the test and enables the conditions for its performance to be standardized. The low cell concentration in the working suspension enables it to be kept on ice without any significant increase in the percentage of dead cells.Department of Microbiology, Smolensk Medical Institute. (Presented by Academician of the Academy of Medical Sciences of the USSR N. N. Zhukov-Verezhnikov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 88, No. 8, pp. 238–239, August, 1979.