缺血后适应对大鼠大脑缺血再灌注脑损伤的S-100B蛋白、超敏感性C反应蛋白表达的影响

目的 探讨缺血后适应对脑缺血大鼠再灌注损伤(IRI)的S-100B蛋白、超敏感性C反应蛋白含量表达的影响,比较在不同时间位点的作用特点.方法 采用线栓法制作大鼠动脉缺血再灌注动物模型.Wistar大鼠随机分为6组:假手术组(Sham组),IRI组,缺血后适应(IP)Ⅰ~Ⅳ组.检测脑损伤标志物S-100B蛋白、超敏感性C反应蛋白(h-CRP)含量变化.结果 与IRI组比较,IPⅠ~Ⅳ各组大鼠神经功能缺损评分、S-100B及C反应蛋白(h-CRP)显著降低,且IPⅠ~Ⅳ各组依次递增,数据均有统计学意义.结论 缺血后适应可改善大鼠术后神经功能缺损评分,下调S-100B蛋白、超敏感性C反应蛋白含量表达,对局灶性脑缺血再灌注损伤大鼠具有保护作用.

Effects of ischemic injury on the expression of S-100B protein and ultra sensitive C reactive protein in cerebral ischemia reperfusion injury in rats

Objective To investigate adaptation ischemic cerebral ischemia- eperfusion injury (ischemia reperfusion injury, IRI) of S-100B protein, ultra- sensitivity C-reactive protein expression comparison sites in different time characteristics.Methods Using suture method cerebral artery occlusion and reperfusion animal model. Wistar rats were randomly divided into six groups: sham operation group (Sham group), IRI group, ischemic postconditioning (ischemia postconditioning, IP)Ⅰ-Ⅳ group. Content change of S-100B protein and super sensitive C reactive protein (h-CRP) in brain injury markers.Results Compared with the IRI group, the neurological deficits score, S-100B and C reactive protein (h-CRP) were significantly decreased in all groups, and the IP I-IV groups were increased in turn. The data were statistically significant.Conclusion After ischemia adaptation can improve the postoperative rats neural function defect score and down regulation of S-100B protein, hypersensitive C reaction protein expression, after focal cerebral ischemia reperfusion injury in rats has a protective effect.