CD44v6在胃癌中的表达及其与幽门螺杆菌感染的关系

背景与目的:目前认为CD44v6在胃癌组织中有较高的表达率,并公认幽门螺杆菌(Helicobactor pylori,HP)感染为Ⅰ类胃癌致癌原,但迄今未见CD44v6与HP关系的文献报道。本文旨在探讨CD44v6在胃癌中的表达及其与HP感染的关系。方法:选取胃癌组织56例、癌旁非癌组织56例、非典型增生胃粘膜32例。CD44v6检测采用RT-PCR与Southern blot法;HP检测采用快速尿酶法与Warthin-Starry银染色;同时用ELISA测定56例胃癌患者血清中HP cagA抗体。结 果:胃粘膜HP阳性组中,转移性胃癌和非转移胃癌的CD44v6表达率分别达100％(12/12)和90.5％(19/21),明显高于癌旁非癌胃组织的48.6％(24/35)与非典型增生胃粘膜的23.6％(6/23);转移性胃癌与非转移胃癌的CD44v6表达率比较无显著差异(P>0.25),非典型增生胃粘膜与胃癌、癌旁非癌胃组织比较有非常显著性差异(P<0.005)。胃粘膜HP阴性组:在转移性胃癌、非转移胃癌、癌旁非癌组织和非典型增生CD44v6表达率分别是66.7％,51.4％,38.1％和18.1％,各组比较均无显著性差异。CD44v6表达与HP感染有密切关系(P<0.01)。肠型胃癌CD44v6表达与HP感染高于弥漫型胃癌(P>0.05)。胃癌患者血清HP cagA抗体阳性率高于胃癌组织HP感染率(P>0.05)。结论:CD44v6在胃癌中有较高的阳性率,与HP感染密切相关,可作为

Expression of CD44v6 in gastric cancer and its correlation with Helicobacter pylori infection

BACKGROUND & OBJECTIVE: it was considered that theexpression of CD44v6 in gastric cancer was high and Helicobacter pylori (HP) infection was type I carcinogen of gastric cancer. So far, no document has been reported about the relationship between HP infection and CD44v6 expression. This study was designed to investigate the relationship between expression of CD44v6 in gastric cancer and HP infection. METHODS: The specimens were divided into three groups: gastric cancer (56 cases), cancer adjacent tissue (56 cases), and gastric mucosa dysplasia (32 cases). The expression of CD44v6 was determined by reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis. HP was determined with the rapid urease test and Warthin-Starry staining. Simultaneously, HP cagA antibody was assayed in the serum of 56 patients with gastric cancer. RESULTS: HP positive infection group: The positive expression rates of CD44v6 in metastasis cancer (100%, 12/12) and non-metastasis cancer (90.5%, 19/21) were higher than those in paracancer tissue (48. 6%, 24/35) and in gastric mucosa dysplasia (23.6% , 6/23) . No statistical difference was found between non-metastasis cancer and metastasis cancer (P> 0.25) . But there were significant differences between gastric mucosa dysplasia and gastric cancer or cancer adjacent tissue ( P <0. 005). HP negative infection group: The positive expression rates of CD44v6 in metastasis cancer, non-metastasis cancer, cancer adjacent tissue, and gastric mucosa dysplasia were 66. 7% , 51. 4% , 35.1%, and 18.1%, respectively; no statistical difference was found between each group. There was a positive correlation between HP infection and CD44v6 expression ( P <0. 01). Both CD44v6 expression and HP infection in intestinal type gastric cancer were higher than those in diffuse type gastric cancer ( P > 0. 05). The positive rate of HP cagA antibody in serum was higher than that of HP infection in gastric cancer tissue ( P > 0. 05) . CONCLUSION: The positive rate of CD44v6 was high in gastric cancer and may serve as a marker for diagnosing gastric cancer. There is obvious correlationship between CD44v6 expression and HP infection.