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枸杞多糖对人前列腺癌PC-3细胞凋亡的影响
引用本文:崔晓燕,罗琼,杨明亮,王丽红,闫俊,李卓能. 枸杞多糖对人前列腺癌PC-3细胞凋亡的影响[J]. 毒理学杂志, 2006, 20(4): 221-223
作者姓名:崔晓燕  罗琼  杨明亮  王丽红  闫俊  李卓能
作者单位:武汉大学公共卫生学院营养与食品卫生系 湖北武汉430071
基金项目:湖北省科技厅科技攻关项目;达能膳食营养研究与宣教基金
摘    要:
目的研究枸杞多糖(LBP)对人前列腺癌PC-3细胞凋亡的影响。方法体外培养的人前列腺癌PC-3细胞经枸(LBP)处理后,采用MTT法测定细胞的增殖情况,流式细胞仪分析LBP对PC-3细胞周期和凋亡的影响,TUNEL法观察PC-3细胞凋亡的变化,用免疫组化法检测其Bcl-2和Bax蛋白的表达。结果LBP可明显抑制人前列腺癌PC-3细胞的生长,并能诱导PC-3细胞凋亡,凋亡率分别为8.5%、17.5%、29.5%、37.5%和41.5%,与对照组比较,差异有非常显著性(P<0.01);同时PC-3细胞Bcl-2/Bax蛋白比值显著下降,呈明显的剂量-效应关系。结论LBP对人前列腺癌PC-3细胞DNA具有损伤作用,能诱导PC-3细胞的凋亡,调节其相关基因的表达。

关 键 词:枸杞多糖  人前列腺癌PC-3细胞  细胞凋亡
文章编号:1002-3127(2006)04-0221-03
收稿时间:2006-01-12
修稿时间:2006-01-12

Effect of lycium barbarum polysaccharides on apoptosis of human prostatic cancer PC-3 cell line
CUI Xiao-yan,LUO Qiong,YANG Ming-liang,WANG Li-hong,YAN Jun,LI Zhuo-neng. Effect of lycium barbarum polysaccharides on apoptosis of human prostatic cancer PC-3 cell line[J]. Journal of Toxicology, 2006, 20(4): 221-223
Authors:CUI Xiao-yan  LUO Qiong  YANG Ming-liang  WANG Li-hong  YAN Jun  LI Zhuo-neng
Affiliation:Department of nutrient and food hygiene, School of Public Health, Wuhan University, Wuhan Hubei 430071,China
Abstract:
Objective The aim of this study was to investigate the influence of lycium barbarum polysaccharides(LBP) on human prostatic cancer cell line PC-3.Methods We cultured human prostate cancer PC-3 cell in vitro and added the LBP to the medium at different concentrations.The MTT assay was used to measure the proliferation of PC-3 cell the influence on the cell cycle and the apoptosis was observed with flow cytometry.TUNEL was used to investigate the apoptosis of PC-3 cell and immunohistochemical technique was used to detect the expression of Bcl-2 and Bax.Results LBP could inhibit the growth of PC-3 cell line,and induced apoptosis and the apoptosis rates were 8.5%,17.5%,29.5%,37.5%,41.5%.Compared to the control group,there were significant differences(P<0.01).the ratio of Bcl-2/Bax protein decreased significantly with a dose-effect relationship.Conclusion LBP inhibits PC-3 cell line in vitro,and the mechanisms may be related to its influence on the cell cycle and inducing of apoptosis.
Keywords:Lycium barbarum polysaccharide  Human prostatic cancer cell  Apoptosis.
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