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| 骨髓间充质干细胞治疗肾缺血再灌注损伤的旁分泌机制 | |
| 引用本文： | 胡红林,王共先,邹丛,黄学明,习小庆,史子敏. 骨髓间充质干细胞治疗肾缺血再灌注损伤的旁分泌机制[J]. 中国组织工程研究与临床康复, 2011, 15(32): 5995-5998. DOI: 10.3969/j.issn.1673-8225.2011.32.024 |
| 作者姓名： | 胡红林 王共先 邹丛 黄学明 习小庆 史子敏 |
| 作者单位： | 1. 南昌大学第二附属医院泌尿外科,江西省南昌市,330006 2. 南昌大学第一附属医院泌尿外科,江西省南昌市,330006 3. 南昌大学第四附属医院内分泌科,江西省南昌市,330003 |
| 基金项目： | 江西省研究生创新专项资金资助 |
| 摘 要： | 背景:骨髓间充质干细胞已用于肾缺血再灌注损伤修复的实验动物研究。目的:探讨骨髓间充质干细胞治疗肾缺血再灌注损伤的旁分泌机制。方法:体外培养、纯化并体外DAPI标记大鼠骨髓间充质干细胞,经下腔静脉移植入肾缺血再灌注损伤模型大鼠体内,观察骨髓间充质干细胞对肾缺血再灌注损伤肾功能的保护作用以及在受体鼠体内的迁移情况,并应用免疫组织化学法检测骨髓间充质干细胞治疗后第2天缺血肾脏组织中血管内皮生长因子、肿瘤坏死因子α细胞因子的表达。结果与结论:与注射生理盐水的对照组比较,细胞移植组大鼠血清肌酐和尿素氮水平在移植后第1、2天明显降低(P<0.05),但细胞移植组移植后第1、2天肾组织中均未见DAPI阳性细胞;第3、4天则逐渐可见DAPI阳性细胞。免疫组织化学染色结果显示,与对照组相比,移植后第2天肾组织中可见较多血管内皮生长因子阳性细胞,而肾组织中肿瘤坏死因子α阳性细胞明显减少。结果显示旁分泌机制参与了骨髓间充质干细胞治疗肾缺血再灌注损伤。 |
| 关 键 词： | 旁分泌机制 骨髓间充质干细胞 肾缺血再灌注损伤 血管内皮生长因子 肿瘤坏死因子α |
Bone marrow mesenchymal stem cells protect against renal ischemia reperfusion injury through paracrine mechanism |
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| Hu Hong-lin,Wang Gong-xian,Zou Cong,Huang Xue-ming,Xi Xiao-qing,Shi Zi-min. Bone marrow mesenchymal stem cells protect against renal ischemia reperfusion injury through paracrine mechanism[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(32): 5995-5998. DOI: 10.3969/j.issn.1673-8225.2011.32.024 | |
| Authors: | Hu Hong-lin Wang Gong-xian Zou Cong Huang Xue-ming Xi Xiao-qing Shi Zi-min |
| Affiliation: | Hu Hong-lin1,Wang Gong-xian2,Zou Cong3,Huang Xue-ming2,Xi Xiao-qing1,Shi Zi-min1 1Department of Urology,the Second Affiliated Hospital of Nanchang University,Nanchang 330006,Jiangxi Province,China,2Department of Urology,the First Affiliated Hospital of Nanchang University,3Department of Endocrinology,the Fourth Affiliated Hospital of Nanchang University,Nanchang 330003 |
| Abstract: | BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) can be used in the animal experiments of renal ischemia reperfusion injury. OBJECTIVE: To explore the paracrine mechamism of BMSCs in treatment of renal ischemia reperfusion injury. METHODS: Rat BMSCs were isolated by percoll density gradient centrifugation and labeled with 4,6-diamidino-2-pheny-lindole (DAPI). Male SD rats were subjected to clamping renal pedicles for 45 minutes and divided randomly into the transplanted group and the control group. DA... |
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