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| 体外诱导羊膜来源间充质干细胞分化为血管内皮细胞 | |
| 引用本文： | 施晶晶,朱栋晓,金伟东,王晓彦,庄瑞娟,李拜红,苗宗宁.体外诱导羊膜来源间充质干细胞分化为血管内皮细胞[J].中国组织工程研究与临床康复,2011,15(36). |
| 作者姓名： | 施晶晶 朱栋晓 金伟东 王晓彦 庄瑞娟 李拜红 苗宗宁 |
| 作者单位： | 1. 无锡市第三人民医院,心内科,江苏省无锡市,214041 2. 无锡市第三人民医院,心超室,江苏省无锡市,214041 3. 无锡市第三人民医院,研究所,江苏省无锡市,214041 |
| 摘 要： | 背景:羊膜来源间充质干细胞植入机体不同类型组织后是否可以分化为相应组织靶细胞呢?目的:检测血管内皮细胞生长因子在体外诱导人羊膜间充质干细胞分化为血管内皮细胞的可行性.方法:分离培养羊膜间充质干细胞,鉴定其表面抗原表达,用含体积分数2%胎牛血清以及50 μg/L血管内皮生长因子的条件培养基诱导,诱导后细胞通过内皮细胞标志物血管内皮生长因子受体2以及v-WF染色鉴定.结果与结论:羊膜间充质干细胞表面抗原CD29、CD44、CD105阳性,CD34、CD45、CD106以及HLA-DR阴性.诱导后细胞形态明显改变,内皮细胞标志物血管内皮细胞生长因子受体2以及v-WF染色结果阳性.提示羊膜间充质干细胞在体外具有分化为血管内皮细胞的能力. |
| 关 键 词： | 羊膜 间充质干细胞 移植 血管内皮细胞 分化 |
In vitro differentiation of human amniotic mesenchymal stem cells into vascular endothelial cells |
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| Shi Jing-jing,Zhu Dong-xiao,Jin Wei-dong,Wang Xiao-yan,Zhuang Rui-juan,Li Bai-hong,Miao Zong-ning.In vitro differentiation of human amniotic mesenchymal stem cells into vascular endothelial cells[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(36). | |
| Authors: | Shi Jing-jing Zhu Dong-xiao Jin Wei-dong Wang Xiao-yan Zhuang Rui-juan Li Bai-hong Miao Zong-ning |
| Institution: | Shi Jing-jing1,Zhu Dong-xiao2,Jin Wei-dong1,Wang Xiao-yan1,Zhuang Rui-juan1,Li Bai-hong1,Miao Zong-ning3 1Department of Cardiology,2Room of Echocardiography,3Institute,Wuxi Third People's Hospital,Wuxi 214041,Jiangsu Province,China |
| Abstract: | BACKGROUND:Whether human amniotic mesenchymal stem cells can differentiate into corresponding target cells after transplantation into different types of tissues? OBJECTIVE:To investigate the feasibility of differentiation from human amniotic mesenchymal stem cells into vascular endothelial cells in vitro induced by vascular endothelial cell growth factor.METHODS:Human amniotic mesenchymal stem cells were isolated,and then characterized by flow cytometry using a panel of monoclonal antibodies.Differentiation... |
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