| 硫酸软骨素对不同时期液氮冷冻保存的家兔心脏瓣膜的影响 |
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| 引用本文： | 吴爱萍,周海英,陈银苹,刘岩,孙影. 硫酸软骨素对不同时期液氮冷冻保存的家兔心脏瓣膜的影响[J]. 第三军医大学学报, 2010, 32(4): 361-363 |
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| 作者姓名： | 吴爱萍 周海英 陈银苹 刘岩 孙影 |
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| 作者单位： | 华北煤炭医学院,病理教研室,河北唐山,063000;河北医科大学附属唐山工人医院胸外科,河北唐山,063000;流行病学与卫生统计学教研室,河北唐山,063000 |
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| 基金项目： | 唐山市临床医学研究计划(08130204A-1)~~ |
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| 摘 要： | 目的观察含硫酸软骨素(chondroitin sulfate,CS)的液氮冷冻保存的同种异体心脏瓣膜在不同保存时间内细胞活性及瓣膜组织学结构的变化。方法取48枚兔主动脉瓣随机分成6组,每组8只并经灭菌处理。对照组于灭菌后立即行瓣膜细胞活性测定及组织学检查;实验Ⅰ～Ⅴ组分别在含有硫酸软骨素的保护液中液氮深低温保存1、3、5、7、9个月后取出,复温后行瓣膜细胞活性测定、光镜及电镜检查。结果实验Ⅰ～Ⅴ组D(490)值分别为(0.073±0.014)、(0.067±0.021)、(0.066±0.015)、(0.063±0.020)、(0.042±0.018),与对照组(0.105±0.029)比较,瓣膜细胞活性明显下降(P<0.05);实验Ⅰ～Ⅳ组间,各组瓣膜细胞活性无明显差异(P>0.05);实验Ⅴ组与实验Ⅰ组比较,瓣膜细胞活性明显下降(P<0.05)。各实验组瓣膜组织学结构保存尚好。结论CS能维持液氮深低温中保存的心脏瓣膜的细胞活性,使冷冻保存7个月内细胞活性无明显下降,组织学结构保存较好,瓣膜可用性大。
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| 关 键 词： | 硫酸软骨素 心脏瓣膜 低温保存时间 细胞活性 |
Chondroitin sulfate protects cardiac valves reserved in cryoprotectant for different periods |
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| Wu Aiping,Zhou Haiying,Chen Yinping,Liu Yan,Sun Ying. Chondroitin sulfate protects cardiac valves reserved in cryoprotectant for different periods[J]. Acta Academiae Medicinae Militaris Tertiae, 2010, 32(4): 361-363 |
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| Authors: | Wu Aiping Zhou Haiying Chen Yinping Liu Yan Sun Ying |
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| Affiliation: | 1Department of Pathology;3Department of Epidemiology and Health Statistics;North China Coal Medical College;Tangshan;Hebei Province;063000;2Department of Thoracic Surgery;Tangshan Workers'Hospital;Hebei Medical University;China |
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| Abstract: | Objective To investigate the pathological changes of cryopreserved homograft cardiac valves in liquid nitrogen with chondroitin sulfate(CS).Methods Forty-eight rabbit aortic valves were randomly divided into control and 5 experiment groups according to different time points(n = 8).All the valves were germicidal treated.The experiment groups were cryopreserved in liquid nitrogen contained 7.5% CS for 1,3,5,7 and 9 months respectively.Then,the activities of cells in different groups were examined and the spec... |
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| Keywords: | chondroitin sulfate heart valves cryopreservation cytoactive |
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