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| 百日咳杆菌外膜蛋白及其组分的毒性与免疫原性 | |
| 引用本文： | 王贤军,吴清明,严杰,方平楚,沈建根,吴兰芳. 百日咳杆菌外膜蛋白及其组分的毒性与免疫原性[J]. 黑龙江医药科学, 1992, 0(5) |
| 作者姓名： | 王贤军 吴清明 严杰 方平楚 沈建根 吴兰芳 |
| 作者单位： | 浙江湖州市第二人民医院，浙江医科大学生物教研室，浙江医科大学生物教研室，浙江医科大学生物教研室，浙江医科大学生物教研室，浙江医科大学生物教研室 |
| 基金项目： | 浙江省自然科学基金资助及浙江省教育委员会基金资助 |
| 摘 要： | 应用Triton X—100抽提和SephadexG—100层析获得百日咳杆菌外膜蛋白(OMP)及其5个组分(OMP_1～OMP_5)。OMP_1～OMP_4均具有促白细胞增多、组胺敏感和胰岛活化蛋白的活性。OMP及其各组分对CHO细胞具有毒性作用,可使该细胞出现聚集生长,~3 H—TdR掺入量下降和核糖体大量脱落及膜旁颗粒减少等现象。OMP和OMP_1～OMP_5免疫原及其抗血清对小鼠的脑内百日咳杆菌感染有较强的主动和被动保护作用。实验结果表明某些OMP组分具有百日咳杆菌毒素的类似毒性,OMP及其组分的毒性与保护作用虽存在一定的关系,但不成正比例。 |
| 关 键 词： | 百日咳杆菌 外膜蛋白 毒性 免疫原性 |
Toxicity and antigenicity of the outer membrane protein and Its fractions of bordetella pertussis |
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| Wang Xianjun. Toxicity and antigenicity of the outer membrane protein and Its fractions of bordetella pertussis[J]. Heilongjiang Medicine and Pharmacy, 1992, 0(5) | |
| Authors: | Wang Xianjun |
| Affiliation: | Wang Xianjun |
| Abstract: | The outer membrane protein(OMP) and its fractions (OMP1 -- OMP5) of B. Pertussis were obtained by extract with Triton X -- 100 and running on a Sephadex G -- 100 column. OMP1 -- OMP4 had LP, HS, and IAP activities. OMP and its fractions showed cytotoxity to CHO cells such as clustesed growth, descent of 3H -- TdR incorporation, falling of the ribosomes and reduction of the membrane particles. OMP, OMP1 -- OMP5 and their antisera possessed strong active and passive protective potentialities in mice when the animals were challenged with B. pertussis. The results showed that some of the fractions had the toxicities similar to PT. OMP and its fractions had stronger antigenicities and protective activities. The relationship existed in the toxicity and protective activity but not show direct proportion. |
| Keywords: | Bordetella pertussis outer membrane protein toxicity protective activity |
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