高效液相色谱-蒸发光散射检测器法测定黄连清胃丸中栀子苷、知母皂苷AⅢ和知母皂苷BⅡ的含量

目的：应用高效液相色谱-蒸发光散射检测器（HPLC—ELSD）法测定黄连清胃丸中栀子苷、知母皂苷AⅢ和知母皂苷BⅡ的含量。方法：采用C18色谱柱（4．6mmX250mm，5μm）；流速为0．9ml／min；乙腈-水（30：70）为流动相；ELSD漂移管温度为90℃；载气（M）流速为2．9SLPM／min。结果：栀子苷、知母皂苷AⅢ和知母皂苷BⅡ进样量分别在0．0618—1．2360μg（r=0．9992）、0．0476～0．9520μg（r=0．9997）和0．1842～3．6840μg（r=0．9993）时进样量的自然对数值与峰面积的自然对数值呈良好的线性关系，平均加样回收率分别为97．67％、98．18％和98．36％，RSD分别为1．09％、1．01％和0．98％（n=6）。结论：本方法测定结果准确、灵敏、重复性好。

Determination of Geniposide,Timosaponin A Ⅲ and Timosaponin B Ⅱ in Huanglian Qingwei Pills by HPLC-ELSD

OBJECTIVE： To develop HPLC-ELSD method for determination of the contents of Geniposide, Timosaponin AⅢ and Timosaponin B Ⅱ in Huanglian Qingwei Pills. METHODS： C18 column （4. 6 mm ×250 mm, 5 μm） was used as the chromatographic column with mobile phase consisting of Acetonitrile-Water （30： 70） at flow rate of 0. 9 ml/min ; the temperature of drift tube was set at 90 ℃ and the flow rate of the cartier gas （ N2 ） was set at 2. 9 SLPM/min. RESULTS： The linear ranges of Geniposide, Timosaponin Am, Timosaponin B II were 0. 061 8-1. 236 0 μg （r =0. 999 2）, 0. 047 6-0. 952 0 μg （r =0. 999 7） and0. 184 2-3. 684 0 μg （r =0. 999 3）, respectively with average recovery rates of 97.67% （RSD = 1.09% ）, 98.18% （RSD = 1.01% ） and 98.3% （RSD =0. 98% ）, respectively. CONCLUSION： The; established method is accurate in determination results, sensitive and reproducible.