|
|||||
|
|
| 一氧化氮供体对实验性自身免疫性脑脊髓炎的作用 | |
| 引用本文： | 尚宏,付锦,王鹏军,王维治. 一氧化氮供体对实验性自身免疫性脑脊髓炎的作用[J]. 中国神经免疫学和神经病学杂志, 2011, 18(2): 79-82. DOI: 10.3969/j.issn.1006-2963.2011.02.002 |
| 作者姓名： | 尚宏 付锦 王鹏军 王维治 |
| 作者单位： | 1. 哈尔滨医科大学附属第四医院神经内科,150001 2. 哈尔滨医科大学附属第二医院神经内科,150086 |
| 基金项目： | 国家自然科学基金，黑龙江省攻关基金 |
| 摘 要： | 目的探讨一氧化氮供体3-吗啉-斯德酮亚胺(3-morpholinosydnonimine,SIN-1)对实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)大鼠的作用。方法应用豚鼠髓鞘碱性蛋白68-86(myelin basic protein 68-86,MBP68-86)主动免疫制作EAE实验动物模型。将大鼠随机分为SIN-1组和对照组,SIN-1组大鼠于致敏后第0~7天给予SIN-1药物干预,动态观察两组大鼠的临床症状及体质量变化,致敏后第14天采用ELISA方法检测各组大鼠单个核细胞(mononuclear cells,MNC)培养上清中γ干扰素(IFN-γ)和白细胞介素-4(IL-4)水平,并观察大鼠脑组织病理变化。结果与对照组比较,SIN-1组大鼠发病时间延迟,恢复时间提前,体质量明显增加,临床症状明显减轻;疾病症状最高评分明显降低。SIN-1组大鼠MNC培养上清中IFN-γ水平为(90.29±9.07)pg/mL,较对照组的(121.57±10.44)pg/mL明显降低(P<0.05);IL-4水平为(18.14±3.98)pg/mL,较对照组的(8.14±1.95)pg/mL明显增加(P<0.05)。SIN-1组大鼠组织病理损伤较对照组明显减轻。结论一氧化氮供体SIN-1可抑制EAE大鼠病情发展,对EAE具有保护作用。 |
| 关 键 词： | 一氧化氮 多发性硬化 实验性自身免疫性脑脊髓炎 |
Effect of 3-morpholinosydnonimine on experimental autoimmune encephalomyelitis |
|
| SHANG Hong,FU Jin,WANG Peng-jun,WANG Wei-zhi. Effect of 3-morpholinosydnonimine on experimental autoimmune encephalomyelitis[J]. Chinese Journal of Neuroimmunology and Neurology, 2011, 18(2): 79-82. DOI: 10.3969/j.issn.1006-2963.2011.02.002 | |
| Authors: | SHANG Hong FU Jin WANG Peng-jun WANG Wei-zhi |
| Affiliation: | SHANG Hong,FU Jin,WANG Peng-jun,WANG Wei-zhi.Department of Neurology,The Second Affiliated Hospital of Harbin Medical University,Harbin Heilongjiang 150086,China |
| Abstract: | Objective To investigate the effect of nitric oxide donor 3-morpholinosydnonimine(SIN-1)on experimental autoimmune encephalomyelitis(EAE)in Lewis rats.Methods EAE was induced by immunization with myelin basic protein 68-86 in Lewis rats.All rats were divided into the SIN-1 group and the control group randomly.SIN-1 was given after the sensitization,starting on the day of sensitization and continued for another 7 days in the SIN-1 group.The clinical symptoms,the body weight changes,pathology changes of brain... |
| Keywords: | nitric oxide multiple sclerosis experimental autoimmune encephalomyelitis |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |