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兔腰椎间盘退行性病变模型的建立及影像学改变
引用本文:赵新刚,石健,侯铁胜. 兔腰椎间盘退行性病变模型的建立及影像学改变[J]. 中国组织工程研究与临床康复, 2008, 12(7): 1376-1379
作者姓名:赵新刚  石健  侯铁胜
作者单位:解放军第二军医大学长海医院骨科,上海市,200433
摘    要:背景:建立最佳的椎间盘退行性病变动物模型对了解椎间盘退行性病变的发生机制、预防与治疗均具有重要意义.目的:以针刺损伤制备兔椎间盘退行性病变模型,通过X射线及MRI分析针刺损伤对椎间盘高度及退行性病变的影响.设计:随机对照观察.单位:解放军第二军医大学长海医院骨科.材料:实验于2005-06/2006-04在解放军第二军医大学长海医院动物中心完成,选用6只健康新西兰大白兔,雌雄不拘,平均6月龄,体质量平均为2.5 kg,均来自解放军第二军医大学长海医院动物中心,许可证号码为SCXK (hu) 2002-0006.实验过程中对动物的处置符合动物伦理学标准.方法:①采用腹膜后入路对实验兔腰椎进行手术,以L3~4椎间盘为正常对照, 不做处置;L4~5椎间盘作为假手术,只进行暴露;L5~6椎间盘暴露后用24 G针头从椎间盘的前外侧针刺3次.②分别于术前及术后4周采用Simens公司CR机拍摄腰椎正侧位X射线片,测量L3~4,L4~5及L5~6椎间隙高度,计算与术前椎间隙高度比值;采用Simens Avanto 1.5 T 医用超导型磁共振扫描仪检测各节段腰椎间盘T2加权信号,根据信号强度记分,4分为正常椎间盘,髓核内信号均匀、明亮;3分为轻微退行性病变椎间盘,T2加权信号部分降低;2分为中度椎间盘退行性病变,T2加权信号明显降低;1分为重度椎间盘退行性病变,其T2加权信号明显降低,而且伴有椎间隙狭窄.主要观察指标:①腰椎X射线侧位片椎间盘高度变化.②椎间盘退行性病变程度.结果:纳入实验兔6只,均进入结果分析,无脱落.①椎间盘高度变化:L3~4及L4~5术后与术前椎间盘高度比分别为0.982 5±0.017 08,0.972 5±0.017 08,均高于L5~6椎间盘高度比(0.550 0±0.025 82),差异有统计学意义(P < 0.01).②腰椎MRI的T2加权像上观察椎间盘退行性病变程度:L3~4及L4~5椎间盘T2加权像信号得分分别为(4.00±0.0),(3.75±0.5)分,均高于L5~6椎间盘,差异有统计学意义(P < 0.01).结论:针刺损伤实验兔椎间盘4周后可造成椎间盘高度降低,并使椎间盘出现明显退行性病变.

关 键 词:腰椎间盘  退行性病变  动物模型  影像学  组织构建  兔腰椎间盘  退行  性病  变模型  影像学改变  changes  models  rabbit  degeneration  lumbar intervertebral disc  Significant  statistical  difference  existed  final  analysis  RESULTS  examination  degree  magnetic resonance imaging
文章编号:1673-8225(2008)07-01376-04
修稿时间:2007-11-16

Establishment of lumbar intervertebral disc degeneration rabbit models and the imageological changes
Zhao Xin-gang,Shi Jian,Hou Tie-sheng. Establishment of lumbar intervertebral disc degeneration rabbit models and the imageological changes[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2008, 12(7): 1376-1379
Authors:Zhao Xin-gang  Shi Jian  Hou Tie-sheng
Abstract:BACKGROUND: The establishment of animal models is of important significance for understanding the pathogenesis, prevention, and treatment of intervertebral disc degeneration. OBJECTIVE: To develop rabbit models of intervertebral disc degeneration by acupuncture and to analyze the effects of acupuncture on intervertebral disc height and degeneration by X-ray and MRI examinations. DESIGN: A randomized and controlled observation. SETTING: Department of Orthopaedics, Changhai Hospital, Second Military Medical University of Chinese PLA. MATERIALS: This study was performed at the Animal Cener, Changhai Hospital, Second Military Medical University of Chonese PLA between June 2005 and April 2006. Six healthy New Zealand white rabbits of either gender, averaging 6 months old, weighing 2.5 kg on average, were provided by Animal Cener, Changhai Hospital, Second Military Medical University of Chinese PLA [permission No. SCXK (hu) 2002-0006], and recruited for this study. The protocol was performed in accordance with ethical guidelines for the use and care of animals.METHODS: ① Operation was made in experimental rabbits via the anterior retroperitoneal approach. The management for the intervertebral discs was as follows: L3-4 was the control disc without exposure or needle stab; L4-5 was subjected to sham-operation with only exposure; L5-6 was the experimental disc with three times of anterior-lateral needle stab after exposure. Lumbar lateral X-ray film was respectively taken with a CR machine (Simens Company) prior to and 4 weeks after surgery for measuring L3-4, L4-5, and L5-6 intervertebral disc height and calculating the ratio with intervertebral disc height prior to surgery. Each segmental lumbar intervertebral disc T2-weighted signal was measured with a 1.5 T medical superconductive magnetic resonance scanner (Simens Avanto). According to signal intensity, intervertebral disc degeneration was graded: a score of 4 indicated normal intervertebral disc without apparent degeneration but with bright signal, a score of 3 indicated slight degeneration with a little decrease in T2-weighted signal, a score of 2 indicated moderate degeneration with apparent decrease in T2-weighted signal, and a score of 1 indicated severe degeneration with apparent decrease in both T2-weighted signal and intervertebral disc height. MAIN OUTCOME MEASURES: Intervertebral disc height change revealed by lumbar lateral X-ray film; Intervertebral disc degeneration degree analyzed through magnetic resonance imaging (MRI) examination. RESULTS: All 6 experimental rabbits were included in the final analysis. The postsurgery-to-presurgery ratio of intervertebral disc height was 0.982 5±0.017 08 and 0.972 5±0.017 08 for L3-4 and L4-5 respectively, which was higher compared to that for L5-6 (0.550 0±0.02 582). Significant statistical difference existed (P < 0.01). T2-weighted singal score was (4.00±0.0) and (3.75±0.5) for L3-4 and L4-5 intervertebral disc, respectively, which was higher compared to that for L5-6 intervertebral disc. Significant statistical difference existed (P < 0.01). CONCLUSION: After 4 weeks of acupuncture, intervertebral disc height is decreased and interterbral disc degeneration obviously appears.
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