地塞米松及甘草酸对小鼠肝细胞凋亡的影响

目的研究地塞米松（ＤＭ）及甘草酸（ＧＬ）对小鼠肝细胞凋亡的影响。方法以肝／体重比、肝组织ＤＮＡ含量、组织学改变及原位凋亡细胞ＴＵＮＥＬ反应为指标，观察ＤＭ（９ｍｇ·ｋｇ－１）及ＧＬ（５０ｍｇ·ｋｇ－１），ｉｐ，每８ｈ１次，对撤除苯巴比妥（ＰＢ）后引起的小鼠肝细胞凋亡的影响。结果与ＰＢ不撤除对照组相比，ＤＭ组小鼠肝／体重比及肝ＤＮＡ含量无明显回落，组织学检查未见明显凋亡改变，ＴＵＮＥＬ反应阴性。ＧＬ组小鼠肝／体重比及ＤＮＡ含量分别回落１２０％和１３６％，肝细胞出现典型凋亡改变，ＴＵＮＥＬ反应阳性。结论ＤＭ能阻断撤除ＰＢ诱导的小鼠肝细胞凋亡，甘草酸对其无影响。

Effects of dexamethasone and glycyrrhizin on the hepatocyteapoptosis caused by phenobarbital withdrawal in mice

AIM To investigate the effects of dexamethasone (DM) and glycyrrhizin (GL) on the hepatocyte apoptosis caused by phenobarbital (PB) withdrawal in mice. METHODS The ratio of liver/body weight, hepatic DNA content, morphological changes and TUNEL reaction (TdTmediated XdUTP nick labeling) as indices of evaluating hepatocyte apoptosis, were used to observe the effects of DM (9 mgkg-1, ip) and GL (50 mgkg-1, ip) on the hepatocyte apoptosis caused by PB withdrawal. RESUITS The regression of hepatic DNA content and ratio of liver/body weight and typical hepatocyte apoptosis, which occured in the control as mice after stopping PB treatment, were not found in the mice treated with DM, and a negative result of TUNEL assay was presented. However, in the mice given GL the ratio of liver/body weight and hepatic DNA content regressed by 120% and 136%, respectively; with a positive reaction of TUNEL assay and the typical changes of hepatocyte apoptosis. CONCLUSION The results showed that DM, but not GL, preve