茶多酚对人鼻咽癌细胞株CNE_2细胞DNA损伤和细胞周期的影响

目的　观察茶多酚（ｔｅａ ｐｏｌｙｐｈｅｎｏｌｓ， ＴＰ） 对人鼻咽癌细胞株ＣＮＥ２ 细胞ＤＮＡ 的损伤作用和细胞周期的影响。方法　用琼脂糖凝胶电泳方法测定ＴＰ对细胞ＤＮＡ的断裂作用；用流式细胞术（ＦＣＭ） 观察ＴＰ对ＣＮＥ２ 细胞周期的变化及其诱导凋亡。结果　ＴＰ可引起ＣＮＥ２ 细胞ＤＮＡ 断裂、其断裂程度随剂量的增加和作用时间的延长而增强。ＴＰ不同浓度和不同作用时间可干扰ＣＮＥ２ 细胞在周期中的进程，使Ｇ１ 期细胞明显增多，Ｓ期细胞减少，细胞分裂增殖指数（ＰＩ）亦随之降低。同时，ＴＰ可诱导ＣＮＥ２ 细胞凋亡，其凋亡率随ＴＰ浓度的增加和作用时间的延长在逐渐增加。结论　ＴＰ１９９８ １２ ０４ 收稿，１９９９ ０７ ３１ 修回＊ 国家自然科学基金资助课题，Ｎｏ ３９３７０８００作者简介：谢冰芬，女，副研究员，硕士生导师，研究方向为肿瘤药理可引起人鼻咽癌细胞株ＣＮＥ２ 细胞的ＤＮＡ 断裂，使细胞停滞于Ｇ１ 期，阻止Ｇ１ 期细胞进入Ｓ期；同时亦可诱导细胞凋亡，这些结果可能为ＴＰ的抗瘤作用提供理论依据

The effects of tea polyphenol on DNA cleavage and cell cycle of human NPC cell line CNE 2 cell

AIM To observe the effect of tea polyphenols (TP) on DNA cleavage and cell cycle of human NPC cell line CNE 2 cells. METHODS To determine cell DNA cleavage with agarose gel electrophoresis technic and to assay CNE 2 cell cycle as well as apoptosis with flow cytometry. RESULTS TP could induce CNE 2 cell DNA cleavage. The degree of DNA cleavage increased with TP concentration increasing and time prolonging of TP treatment. Different TP concentrations and different time of TP treatment could disturb different progression of CNE 2 cells at cycle, also could make G 1 phase cell markedly increasing and S phase cells decreasing. Therefore, the cell proliferating index (PI) also decreased. At the same time, TP could induce CNE 2 cell apoptosis. The cell apoptosis rates increase with TP concentration increasing and time prolonging of TP treatment. CONCLUSION TP could induce DNA cleavage of human NPC cell line CNE 2 cells, also could make cells stagnate at G 1 phase and prevent G 1 phase cells to come into S phase. At the same time, TP could induce CNE 2 cell apoptosis. The results will provide theoretical basis for the antitumor action mechanism of TP.