氯通道阻断剂对T淋巴细胞增殖的影响

目的探讨氯通道开放在Ｔ淋巴细胞增殖过程中的作用。方法新鲜分离成人外周血Ｔ淋巴细胞，以ＣｏｎＡ诱导其增殖，用［３Ｈ］ ＴｄＲ参入法测定并比较氯通道阻断剂ＤＩＤＳ、ＮＰＰＢ、９ ＡＣ、ＮＦＡ、Ｆｕｒｏｓｅｍｉｄｅ对Ｔ淋巴细胞增殖的作用；测定钙通道阻滞剂ＳＫ＆Ｆ９６３６５、硝苯吡啶以及免疫抑制剂环孢霉素Ａ（ＣｓＡ）对Ｔ淋巴细胞增殖的作用；比较ＤＩＤＳ、ＮＰＰＢ与ＳＫ＆Ｆ９６３６５或ＣｓＡ的相互作用。结果９ ＡＣ，ＮＦＡ，Ｆｕｒｏｓｅｍｉｄｅ对Ｔ细胞增殖无明显抑制作用；ＤＩＤＳ、ＮＰＰＢ呈浓度依赖性地抑制Ｔ细胞增殖，ＩＣ５０分别为１６８２３±３３６和（１２４３５±４２４）μｍｏｌ·Ｌ－１，并显著增强ＳＫ＆Ｆ９６３６５及ＣｓＡ抑制Ｔ细胞增殖的作用，且ＮＰＰＢ增强抑制作用强于ＤＩＤＳ。结论阻断氯通道可抑制ＣｏｎＡ诱导的Ｔ淋巴细胞增殖活化，氯通道开放影响细胞钙运动。

Effects of chloridechannel blockers on T lymphocyte proliferation

AIM To study the function of chloride channel on the proliferation of human T lymphocyte. METHODS With thymidine incorporation, the effects of chloride channel blockers, nifedipine SK&F96365 and cyclosporin A(CsA) on Con A induced human T lymphocyte proliferation are measured. The interaction between chloride channel blockers and SK&F96365 or CsA is also verified. RESULTS DIDS and NPPB inhibited human T lymphocyte proliferation in a concentrationdependent fashion with the IC50 value of 16823336 and (12435424) molL-1for DIDS and NPPB respectively, whereas 9AC NFA furosemide have no significant effect. On the concentration of 25 molL-1, DIDS or NPPB did not evoke significant effect. However, they significantly enhanced the inhibitory effects of SK&F96365 (10 molL-1) or CsA(100 nmolL-1). THZCONCLUSIONS The chloride channel blockers can inhibit the Con Ainduced T lymphocyte proliferation and potential the effects of SK&F96365 and CsA. These data suggest that chloride channel blockers may play the role by different mechanism from that of SK&F96365 and CsA.