用紫外-可见分光光度法测定感冒安颗粒中总酚酸的含量

目的：采用紫外-可见分光光度法测定感冒安颗粒中总酚酸的含量,并优化超声提取感冒安颗粒中总酚酸的工艺条件。方法：以没食子酸为对照品,在760nm处用紫外-可见分光光度法测定总酚酸的含量。考察甲醇浓度、甲醇用量和超声提取时间3个因素对感冒安颗粒中总酚酸提取率的影响,用L9（34）正交设计确定最佳超声提取条件。结果：感冒安颗粒中总酚酸的最佳提取工艺为用35ml 50%甲醇超声提取30min。没食子酸浓度在10～60μg/ml范围内与吸光度呈良好线性关系（r=0.999 8）。用紫外-可见分光光度法测定感冒安颗粒中总酚酸含量的加样回收率为（100.72±2.53）%（n=9）,精密度、重复性和稳定性良好。结论：该方法简便易行,稳定性好,对提高感冒安颗粒的质量控制标准具有参考意义。

Determination of total phenolic acid content in Ganmao'an granules by ultraviolet-visible spectrophotometry

Objective： To determine the content of total phenolic acid in Ganmao＇ an granules by ultraviolet-visible （UV-VIS） spectrophotometry and to optimize the ultrasonic extraction technology of total phenolic acid from Ganmao＇ an granules. Methods： The content of total phenolic acid was determined at 760 nm by UV-VIS spectropbotometry. Gallic acid was used as the control. Ultrasonic extraction technology was optimized by L9 （34） orthogonal design, with methanol concentration, methanol volume and extraction time as 3 influential factors. Results： The optimal extraction technology of total phenolic acid from Ganmao＇an granules was using 35 ml 50% methanol as solvent and ultrasonic extraction for 30 min. The concentration of gallic acid displayed good linearity within the range of 10-60μg/ml（r=0. 999 8）. The recovery of total phenolic acid determined by UV-VIS spectrophotometry was （100.72±2. 53） % （n = 9）, with good precision, reproducibility and stability. Conclusion： The method is simple,stable and useful for enhancing the quality control standards of Ganmao＇an granules.