Phase Diagrams Map the Properties of Antiviral Agents Directed against Hepatitis B Virus Core Assembly |
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| Authors: | Lichun Li Srinivas Reddy Chirapu M. G. Finn Adam Zlotnick |
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| Affiliation: | aDepartment of Molecular and Cellular Biochemistry, Indiana University, Bloomington, Indiana, USA;bDepartment of Chemistry and Biochemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California, USA |
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| Abstract: | Assembly effectors are small molecules that induce inappropriate virus capsid assembly to antiviral effect. To identify attributes of hepatitis B virus (HBV) assembly effectors, assembly reaction products (normal capsid, noncapsid polymer, intermediates, and free dimeric core protein) were quantified in the presence of three experimental effectors: HAP12, HAP13, and AT-130. Effectors bound stoichiometrically to capsid protein polymers, but not free protein. Thermodynamic and kinetic effects, not aberrant assembly, correlate with maximal antiviral activity. |
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