miR-429在鼻咽癌细胞中的表达及其对细胞增殖、迁移的影响

目的:探讨微小RNA-429(miR-429)在鼻咽癌细胞中的表达及其对细胞增殖、迁移及侵袭能力的影响.方法:选取本院收治的78例鼻咽癌患者为鼻咽癌组,另选取同期因其他疾病需进行鼻内镜手术患者33例为对照组.采用实时荧光定量PCR(qRT-PCR)法检测两组组织中miR-429的表达.体外培养人鼻咽上皮细胞NP69与鼻...

The expression of miR-429 in nasopharyngeal carcinoma cells and its effects on cell proliferation and migration

Objective:To investigate the expression of microRNA-429(miR-429)in nasopharyngeal carcinoma cells and its effects on cell proliferation,migration and invasion.Methods:78 patients with nasopharyngeal carcinoma were selected as nasopharyngeal carcinoma group,and 33 patients who underwent endoscopic sinus surgery for other diseases were selected as control group.The expression of miR-429 in tissues of two groups was detected by real-time fluorescence quantitative PCR(qRT-PCR).Human nasopharyngeal epithelial cell line NP69 and nasopharyngeal carcinoma cell line CNE-2 were cultured in vitro.miR-429 negative control plasmid and miR-429 mimics plasmid were transfected into CNE-2 cells and named as negative transfection group and miR-429 overexpression group respectively.The untreated cells were named blank group.The expression of miR-429 in cells of each transfected group was detected by qRT-PCR.MTT assay was used to detect the proliferation of each transfection group.Cell colony formation was detected by plate clone cell formation experiment.Transwell was used to detect cell migration and invasion.The expressions of E-box binding zinc finger protein(ZEB1),matrix metalloproteinase-2(MMP-2)and calcium adhesion protein E(E-cadherin)were detected by Western blot.Results:The expression of miR-429 in nasopharyngeal carcinoma tissues was significantly lower than that in control group(P<0.05),and the expression of miR-429 in CNE-2 cells was significantly lower than that in NP69 cells(P<0.05).The expression level of miR-429 in miR-429 overexpression group was significantly higher than that in blank group and negative transfection group(P<0.05).The proliferation ability of miR-429 overexpression group was significantly lower than that of blank group and negative transfection group(P<0.05).The numbers of colonies,migrating cells and invasive cells in the overexpression group were significantly lower than those in the blank group and negative transfection group(P<0.05).The expressions of ZEB1 and MMP-2 proteins in the overexpression group were significantly lower than those in the blank group and negative transfection group,while the expression of E-cadherin protein was significantly higher(P<0.05).Conclusion:The expression of miR-429 in nasopharyngeal carcinoma is low,overexpression of miR-429 can significantly inhibit the proliferation,migration and invasion of nasopharyngeal carcinoma cells.