山苦荬保肝活性部位筛选及其化学成分研究

目的:筛选山苦荬保肝活性部位并对其化学成分进行研究.方法:采用系统溶剂法,以70％乙醇对山苦荬药材进行提取,用不同溶剂对所得提取物进行萃取,分别得石油醚、乙酸乙酯、正丁醇部位和剩余水部位.以对乙酰氨基酚诱导人肝细胞HL-7702复制肝损伤细胞模型,采用MTT法检测上述各部位(40μg/mL,均以生药量计)对损伤细胞的保...

Screening of Hepatoprotective Activity Fractions from Ixeris chinensis and Study on Its Chemical Compositions

OBJECTIVE:To screen the hepatoprotective active fractions from Ixeris chinensis and study its chemical constituents.METHODS:The petroleum ether,ethyl acetate,n-butanol and residual water fractions from 70%ethanol extract of I.chinensis were extracted by systematic solvent method.Human hepatocytes HL-7702 were induced by acetaminophen to induce liver injury model.MTT method was used to detect the protective effect of the above fractions(40μg/mL,by the dosage of crude drug)on injured cells,and the active fractions were screened.The active fractions were separated and purified by silica gel column and Sephadex column chromatography.The structure of the compounds were identified by physical and chemical properties and spectral data(hydrogen spectrum,carbon spectrum).RESULTS:After treated with different fractions of I.chinensis,the cell survival rate of each administration group was increased significantly,compared with model group(P<0.01),and the n-butanol and water fractions had the strongest activity(the cell survival rates were 49.3%and 52.2%,respectively).Six compounds were isolated from n-butanol fraction and identified as sonchifolignan A(Ⅰ),apigenin-7-O-β-D-glucopyranoside methyl ester(Ⅱ),luteolin-7-O-β-D-glucuronopyranoside methyl ester(Ⅲ),luteolin-7-O-β-D-glucopyranoside(Ⅳ),apigenin-7-O-β-Dglucopyranoside(Ⅴ)and luteolin(Ⅵ).CONCLUSIONS:The n-butanol fraction is regarded as an effective position for protecting liver,and flavonoids are the main active components.