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| FilmArray脑炎/脑膜炎多重病原体核酸联检试剂盒在中枢神经系统感染病原学诊断中的价值 | |
| 引用本文： | 张巧珍,罗丽娟,王翠锦,陶悦,干驰,曹清. FilmArray脑炎/脑膜炎多重病原体核酸联检试剂盒在中枢神经系统感染病原学诊断中的价值[J]. 中国小儿急救医学, 2021, 0(3): 161-164 |
| 作者姓名： | 张巧珍 罗丽娟 王翠锦 陶悦 干驰 曹清 |
| 作者单位： | 上海交通大学医学院附属上海儿童医学中心感染科;上海交通大学医学院附属上海儿童医学中心神经内科;上海交通大学医学院附属上海儿童医学中心转化医学研究所 |
| 基金项目： | 上海交通大学医学院转化医学协同创新中心合作研究项目(TM201820;TM201927)。 |
| 摘 要： | 目的:探讨FilmArray脑炎/脑膜炎多重病原体核酸联检试剂盒(FilmArray ME Panel)在中国儿童中枢神经系统感染病原学诊断方面的应用价值。方法:收集2019年3月至11月上海交通大学医学院附属上海儿童医学中心收治的145例疑诊中枢神经系统感染患儿的经腰椎穿刺获得的脑脊液标本，对所有患儿的脑脊液标本行培... |
| 关 键 词： | 中枢神经系统感染 儿童 病原体 快速诊断 分子诊断技术 |
Clinical value of FilmArray meningitis/encephalitis Panel in detecting the etiology of infection in central nervous system |
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| Zhang Qiaozhen,Luo Lijuan,Wang Cuijin,Tao Yue,Gan Chi,Cao Qing. Clinical value of FilmArray meningitis/encephalitis Panel in detecting the etiology of infection in central nervous system[J]. Chinese Pediatric Emergency Medicine, 2021, 0(3): 161-164 | |
| Authors: | Zhang Qiaozhen Luo Lijuan Wang Cuijin Tao Yue Gan Chi Cao Qing |
| Affiliation: | (Infection Department,Shanghai Children′s Medical Center,Shanghai Jiaotong University,Shanghai 200127,China;Neurology Department,Shanghai Childrens Medical Center,Shanghai Jiaotong University,Shanghai 200127,China;Institute of Translational Medicine,Shanghai Children′s Medical Center,Shanghai Jiaotong University,Shanghai 200127,China) |
| Abstract: | Objective To explore the value of FilmArray meningitis/encephalitis(ME)Panel in etiological diagnosis of infection in central nervous system(CNS)in Chinese children.Methods Cerebrospinal fluid(CSF)obtained through lumbar puncture was collected from 145 patients with suspected CNS infection at Shanghai Children′s Medical Center Affiliated to Shanghai Jiaotong University School of Medicine from March 2019 to November 2019.All specimens were cultured simultaneously,which were detected by FilmArray ME Panel,and the results of cerebrospinal fluid culture and FilmArray ME Panel were compared.Results Among 145 patients with suspected CNS infection,three samples were found to be positive after cerebrospinal fluid culture,and the positive rate was 2.1%(3/145).For the FilmArray ME Panel,30 specimens were found to be positive,with a positive rate of 20.7%(30/145),and the difference of positive rate between the two methods was statistically significant(χ2=24.927,P<0.05).Among the samples FilmArray ME Panel tested positive with pathogen,26 specimens were positive with virus making up 17.9%(26/145)and enterovirus(15.2%)was the primary pathogen.In addition,of the 142 specimens cerebrospinal fluid culture negative,28 samples were tested positive by the FilmArray ME Panel,accounting for 19.7%(28/142).Conclusion FilmArray ME Panel has the characteristics with high positive rate and could be time-saving.Meanwhile,FilmArray ME Panel has significant advantage in the detection of virus and improves the positive detection rate of virus. |
| Keywords: | Central nervous system infection Children Pathogen Rapid diagnosis Molecular diagnostic techniques |
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