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Alkaline phosphatase grafting on bioactive glasses and glass ceramics |
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| Authors: | Enrica Verné Sara Ferraris Chiara Vitale-Brovarone Silvia Spriano Claudia L. Bianchi Alberto Naldoni Marco Morra Clara Cassinelli |
| Affiliation: | 1. School of Stomatology, Lanzhou University, Lanzhou 730000, PR China;2. State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China;3. Liaocheng People’s Hospital, Medical school of Liaocheng University, Shandong 252000, PR China;4. Institute of Sensing Technology, Gansu Academy of Sciences, Lanzhou 730000, PR China;1. University of Rennes 1, UMR CNRS 6226, Campus de Beaulieu, 35042 Rennes, France;2. Animal Ecophysiology Laboratory, Sfax Faculty of Science, Department of Life Sciences, Sfax, Tunisia;3. Science Materials and Environement laboratory, Sfax Faculty of Science, Sfax, Tunisia;4. Histology, Orthopaedic and Traumatology laboratory Sfax Faculty of Medicine Sfax, Tunisia;1. Institute of Materials Physics and Engineering, Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy;2. Department of Biomedical Sciences/Center for Clinical Examination Practicum Support, Chubu University, 1200 Matsumoto cho, Kasugai, Japan;1. Graduate Program in Chemical Engineering (POSENQ), Federal University of Santa Catarina (UFSC), 88040-900 Florianópolis, SC, Brazil;2. Graduate Program in Materials Science and Engineering (PGMAT), Federal University of Santa Catarina (UFSC), 88040-900 Florianópolis, SC, Brazil;3. Laboratory of Porous Media and Thermophysical Properties (LMPT), Department of Mechanical Engineering (EMC), Federal University of Santa Catarina (UFSC), 88040-900 Florianópolis, SC, Brazil |
| Abstract: | Bone integration of orthopaedic or dental implants and regeneration of damaged bone at the surgical site are still unresolved problems in prosthetic surgery. For this reason, biomimetic surfaces (i.e. both inorganic and biological bioactive surfaces) represent a challenge for bone implantation. In this research work a hydrolase enzyme (alkaline phosphatase) was covalently grafted to inorganic bioactive glass and glass ceramic surfaces, in order to impart biological bioactivity. The functionalized samples were analysed by means of X-ray photoelectron spectroscopy in order to verify enzyme presence on the surface. Enzyme activity was measured by means of UV–visual spectroscopy after reaction with the natural substrate. Scanning electron microscopy–energy-dispersive spectroscopy observations allowed monitoring of the morphological and chemical modification of the materials during the different steps of functionalization. In vitro inorganic bioactivity was investigated by soaking samples in simulated body fluid. Enzymatic activity of the samples was tested and compared before and after soaking. Enzymatic activity of the solution was monitored at different experimental times. This study demonstrates that alkaline phosphatase could be successfully grafted onto different bioactive surfaces while maintaining its activity. Presence of the enzyme in vitro enhances the inorganic bioactivity of the materials tested. |
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