Polylactide-glycoli acid and rapamycin coating intraocular lens prevent posterior capsular opacification in rabbit eyes |
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Authors: | Hongling Liu Lan Wu Shaoying Fu Yongsheng Hou Ping Liu Hao Cui Jingjing Liu Lin Xing Xiaomei Zhang |
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Institution: | (1) Department of Ophthalmology, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Str, Nangang District, Harbin, Heilongjiang Province, 150001, People’s Republic of China |
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Abstract: | Objectives Posterior capsular opacification (PCO) is caused by the proliferation and migration of residual lens epithelium cells (LECs)
after extracapsular cataract extraction (ECCE). Rapamcin (RAPA) is known to be a potent immunosuppressive drug with anti-inflammatory
and anti-proliferative effects. The aim of this study was to investigate the safety and efficacy of rapamycin sustained release
from modified intraocular lens (IOLs) in the prevention of PCO in rabbits.
Methods Three types of IOLs were used, including the original IOL without modification, IOL with polylactide-glycoli acid (PLGA) coating
(PLGA-IOL), and RAPA-loaded PLGA-IOL (RAPA-PLGA-IOL). Sixty New Zealand albino rabbits undergoing phacoemulsification in left
eyes were randomly and equally divided into three groups. Group A was implanted with the original IOLs, group B was implanted
with the PLGA-IOLs, and group C was implanted with the RAPA-PLGA-IOLs. All of the 60 treated left eyes were examined by a
slit-lamp microscope. The concentrations of RAPA in the aqueous humor and blood were determined by high-performance liquid
chromatography (HPLC), indicating an vivo release of drug from the polymer carrier. Anterior segment tissue was histologically
examined, and wet posterior capsules were weighed. Six months after intervention the PCO was graded.
Results The mean concentrations of RAPA in the aqueous humor from group C at 2 h, 1 days, 3 days, and 7 days after operation were
12.81 ± 1.27 μg/ml, 14.57 ± 0.99 μg/ml, 6.39 ± 0.95 μg/ml, and 1.10 ± 0.32 μg /ml respectively. The concentrations of RAPA
in blood were undetectable. During the early days after the operation, the reactions of the anterior chamber from groups A
and B were more severe than from group C. Our findings showed that the initial appearance of PCO in group C was much later
than in the other two groups. The wet posterior capsules were weighed to be 0.3735 ± 0.0943 g (group A), 0.3754 ± 0.1093 g
(group B), and 0.0432 ± 0.0089 g (group C). Histological observation showed a similar phenomenon, that there was remarkably
less accumulation of lens materials on the posterior capsules in group C than in the other two groups.
Conclusion Our findings suggest that the designed RAPA-PLGA-IOL effectively prevented formation and development of PCO for a relatively
long duration. |
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Keywords: | Posterior capsule opacification Rapamycin Intraocular lens Polylactide-glycoli acid (PLGA) |
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