| 聚合酶链反应单链构象多态性检测乳腺癌中P53基因突变 |
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| 引用本文： | 林汝仙,孙志贤.聚合酶链反应单链构象多态性检测乳腺癌中P53基因突变[J].解放军医学杂志,1994,19(5):339-341. |
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| 作者姓名： | 林汝仙 孙志贤 |
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| 作者单位： | 军事医学科学院 |
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| 摘 要： | 应用聚合酶链反应单链构象多态性方法，对２４例原发性乳腺癌肿瘤组织基因组ＤＮＡ进行了分析，结果表明：其中７例存在Ｐ５３基因的突变，突变频率约为３０％。同时对其中１０例进行Ｓｏｕｔｅｒｎｂｌｏｔ分析，有２例在ｃｈｒ１７Ｐ上存在等位基因的缺失，而其另一等位基因上均存在基因突变。
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| 关 键 词： | 聚合酶链反应 乳腺癌 P53 基因突变 |
USk OF POLYMERASE CHAIN REACTION SINGLE STRAND STRAND CONFORMATIONPOLYMORPHISM TECHNIQUE TO DETECT p53 GENE MUTATATIONS IN BREASTCANCERS |
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| Lin Ruxian,Sun Zhixian , Dang Jinjun ,et al..USk OF POLYMERASE CHAIN REACTION SINGLE STRAND STRAND CONFORMATIONPOLYMORPHISM TECHNIQUE TO DETECT p53 GENE MUTATATIONS IN BREASTCANCERS[J].Medical Journal of Chinese People's Liberation Army,1994,19(5):339-341. |
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| Authors: | Lin Ruxian Sun Zhixian Dang Jinjun |
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| Institution: | Lin Ruxian;Sun Zhixian ; Dang Jinjun ; et al.The Academy of Military Medical Sciences Beijing 100850 |
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| Abstract: | We utilized the polymerase chain reaction single strand conformation polymorphism(PCR-SSCP ) technique to detect twentyfour primary breast tumors mutations of p53 including exons 5-9, theresults indicated that seven mutations in the p53 gene were detected in 24 tumors(30%).Loss of het-erozygosity on chromosome 17P was detected by southern blot in two of ten tumor DNAs, the two tumorDNAs all contained mutations in the remaining allese |
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| Keywords: | PCR-SSCP Breast cancer p53 Gene |
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