年龄对小鼠腹腔巨噬细胞TLR2/4表达水平及功能的影响

目的：观察不同年龄组小鼠腹腔巨噬细胞Toll样受体2/4（TLR2/4）表达水平的差别,以及该细胞在脂多糖（LPS）刺激下,炎症因子TNF-α分泌水平的差别。方法：采用real-time PCR和流式细胞技术,检测低龄组和高龄组小鼠腹腔巨噬细胞TLR2/4 mRNA和蛋白表达水平的差异;同时检测1μg/mL大肠杆菌（E.coli）LPS或1mg/L牙龈卟啉单胞菌（P.gingivalis）LPS刺激后,两组细胞TLR2/4表达水平的变化;采用ELISA技术检测炎症因子TNF-α分泌水平的变化。结果：刺激前,两组细胞TLR2/4 mRNA和蛋白表达水平均无明显差别。E.co-li LPS或P.gingivalis LPS刺激后,低龄组细胞TLR2/4mRNA和蛋白表达水平均明显高于高龄组（P〈0.05）,其分泌的TNF-α水平也显著高于高龄组（P〈0.05）。结论：年龄对小鼠腹腔巨噬细胞TLR2/4表达水平没有显著影响,但增龄性变化可能导致TLR2/4功能的减退。

Eiffects of Age on the Exnression and Function of TLR2/4 in Murine Peritoneal Macrophages.

Objective： To observe the effect of age on the expression and function of Toll-like receptor 2/4（TLR2/4） in murine peritoneal macrophages.Methods： TLR2/4 mRNA and protein expression were detected in young and aged-murine peritoneal macrophages by real-time PCR and FACS.In addition,TLR2/4 expressions were also determined in these cells stimulated with 1μg/ml E.coli lipopolysaccharides（LPS） or Porphyromonas gingivalis（P.gingivalis） LPS.The levels of TNF-α were investigated by ELISA.Results： There were no significant differences in TLR2/4 mRNA and protein expression in young and aged-murine peritoneal macrophages.However,the higher expressions of TLR2/4 and the increased amounts of TNF-α were observed in young peritoneal macrophages stimulated with 1μg/ml E.coli LPS or P.gingivalis LPS（P0.05）.Conclusion： There were no effects of age on the expression of TLR2/4 in murine peritoneal macrophages,but there were impaired functions of TLR2/4 in aged-mice.