新型根管封闭剂对MC3T3-E1成骨细胞的细胞毒性评估

目的：评估新型根管封闭剂RealSeal sealer、GuttaFlow对MC3T3-E1成骨细胞的细胞毒性,并与传统的AH Plus比较。方法：制备RealSeal sealer、GuttaFlow和AH Plus（固化7d）的材料浸提液,倍比稀释为4种浓度：100%、50%、25%、12.5%;MC3T3-E1细胞于其中分别培养24h和72h,CCK-8法检测细胞存活率,评价不同材料的细胞毒性。结果：在实验期内,RealSeal sealer组的细胞存活率显著低于AH Plus、GuttaFlow（P〈0.05）,AHPlus、GuttaFlow和阴性对照组间无显著差异。结论：在本实验条件下,RealSeal sealer对MC3T3-E1成骨细胞的毒性最强,而GuttaFlow和AH Plus几乎无细胞毒性。

Cytotoxicity of New Root Canal Sealers on MC3T3 -E1 Osteoblast Cells.

Objective： To investgate the cytotoxicity of new root canal sealers,RealSeal Sealer and GuttaFlow,on MC3T3-E1 osteoblast cells and compare with traditional material AH Plus.Methods： Six samples of each sealer were fabricated in sterile cylindrical Teflon tubes and were kept in a humid chamber at 37℃ for 7 days.Then extraction of the specimens was obtained after incubating with cell culture medium for 3 day.MC3T3-E1 cells were cultured with different concentrations of the extracts（100%,50%,25%,and 12.5%）,and the cell relative proliferation rate was evaluate by CCK-8 assay at 24h and 72 h.Results： RealSeal Sealer showed more cytotoxicity to MC3T3-E1 cells than AH Plus and GuttaFlow（P0.05） within 3 days.The cytotoxicity of GuttaFlow was similar to AH Plus and the negative control.Conclusion： RealSeal Sealer had a strong cytotoxicity on MC3T3-E1cells,while GuttaFlow and AH Plus showed almost nontoxicity.