| 正负向磁性细胞分选技术纯化血小板方法的比较 |
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| 引用本文： | 张印则,熊文,邹红岩,陈佐伟,张艳艳,周丹,李桢,邵超鹏,卢亮. 正负向磁性细胞分选技术纯化血小板方法的比较[J]. 中国输血杂志, 2007, 20(2): 96-98 |
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| 作者姓名： | 张印则 熊文 邹红岩 陈佐伟 张艳艳 周丹 李桢 邵超鹏 卢亮 |
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| 作者单位： | 1. 深圳市血液中心,广东深圳,518035
2. 深圳市人民医院核医学科 |
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| 摘 要： | 目的获得纯化的血小板,为血小板免疫功能的研究提供实验基础。方法应用磁性细胞分选技术纯化血小板;流式法评价纯化后血小板活化率;白细胞绝对计数法评价有核细胞清除率。结果分选前血小板基础活化率为(2.39±1.10)%,负向分选后为(2.56±1.08)%,正向分选后为(16.76±4.04)%;正、负向分选的有核细胞清除率分别为(98.44±0.24)%及(98.47±0.18)%。正负向分选后血小板回收率分别为(76.5±1.49)%及(79.2±2.61%)。结论磁性细胞负向分选技术适合进行血小板纯化。
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| 关 键 词： | 磁性细胞分选 血小板 纯化 |
| 文章编号： | 1004-549X（2007）02-0096-03 |
| 收稿时间： | 2006-09-04 |
| 修稿时间： | 2006-09-042007-05-15 |
Study of positive and negative magnetic activated cell sorting separation of platelet purification |
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| ZHANG Yinze,XIONG Wen,ZOU Hongyan,et al.. Study of positive and negative magnetic activated cell sorting separation of platelet purification[J]. Chinese Journal of Blood Transfusion, 2007, 20(2): 96-98 |
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| Authors: | ZHANG Yinze XIONG Wen ZOU Hongyan et al. |
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| Affiliation: | ZHANG Yinze,XIONG Wen,ZOU Hongyan,et al.Shenzhen Blood Center,Shenzhen 518035,China |
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| Abstract: | Objective To gain purified platelets for further research of the immune function of platelet.Methods Platelets were purified by positive(MS) and negative(LD) magnetic cell sorting(MACS) separation.The percentage of activated platelets was detected by flow cytometry and nucleated cell clearance was evaluated by white cell count.Results The percentage of activated platelets before separation was(2.39±1.10)%,and increased to(2.56±1.08)% and(16.76±4.04)%,respectively,after MS and LD MACS.The clearances of nucleated cells after MACS MS and LD were(98.44±0.24)% and(98.47±0.18)%,respectively.The recovery rate of purified platelet after MS and LD was(76.50±1.49)% and(79.20±2.61)%.Conclusion MACS LD method was more suitable for the platelet purification. |
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| Keywords: | Magnetic activated cell sorting(MACS) Platelet Purification |
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