首页 | 本学科首页   官方微博 | 高级检索  
     

丙烯醛对DNA分子的损伤作用
引用本文:袭著革,晁福寰,杨丹凤,孙咏梅,李官贤,张华山,张伟,杨玉花,刘焕亮. 丙烯醛对DNA分子的损伤作用[J]. 环境与健康杂志, 2004, 21(5): 293-295
作者姓名:袭著革  晁福寰  杨丹凤  孙咏梅  李官贤  张华山  张伟  杨玉花  刘焕亮
作者单位:军事医学科学院卫生学环境医学研究所,天津,300050;军事医学科学院卫生学环境医学研究所,天津,300050;军事医学科学院卫生学环境医学研究所,天津,300050;军事医学科学院卫生学环境医学研究所,天津,300050;军事医学科学院卫生学环境医学研究所,天津,300050;军事医学科学院卫生学环境医学研究所,天津,300050;军事医学科学院卫生学环境医学研究所,天津,300050;军事医学科学院卫生学环境医学研究所,天津,300050;军事医学科学院卫生学环境医学研究所,天津,300050
基金项目:国家科技部“十五”社会公益基金资助(2002DIA10001),国家科技部“十五”攻关项目资助(2001BA704B01)
摘    要:目的 通过研究丙烯醛对DNA分子的损伤,探讨丙烯醛的遗传毒性效应及其分子机制。方法 应用单细胞凝胶电泳技术检测丙烯醛引起的DNA断裂、DNA交联以及DNA.蛋白质交联;应用液相色谱.电化学法研究丙烯醛致DNA分子产生氧化损伤标志物8-羟基脱氧鸟苷(8-OHdG)。结果 丙烯醛可诱导人外周血淋巴细胞DNA发生链断裂,但不引起DNA-DNA、DNA-蛋白质交联;丙烯醛与小牛胸腺DNA的体外作用较弱,但在铁离子介导下对DNA的氧化能力增强。可产生一定量的8-OHdG加合物;动物实验表明丙烯醛诱导大鼠肺组织DNA氧化损伤生成少量8-OHdG。结论 丙烯醛具有直接的遗传毒性效应,产生自由基造成DNA氧化损伤是其遗传毒性效应的主要途径。

关 键 词:DNA损伤  丙烯醛  遗传毒性  8-羟基脱氧鸟苷
文章编号:1001-5914(2004)05-0293-03
修稿时间:2003-09-15

Study on the Effects of DNA Damage Induced by Acrolein
XI Zhu,ge,CHAO Fu,huan,YANG Dan,feng,et al.. Study on the Effects of DNA Damage Induced by Acrolein[J]. Journal of Environment and Health, 2004, 21(5): 293-295
Authors:XI Zhu  ge  CHAO Fu  huan  YANG Dan  feng  et al.
Affiliation:XI Zhu,ge,CHAO Fu,huan,YANG Dan,feng,et al. Institute of Health and Environmental Medicine,Academy of Military Medical Sciences,Tianjin 300050,China
Abstract:Objective To study the genotoxicity and its molecular mechanism of the typical environmental pollutant,acrolein through the studying on DNA damage effects induced by acrolein. Methods DNA strand breakage, DNA,DNA crosslinks and DNA,protein crosslinks induced by the acrolein were detected with single cell gel electrophoresis (SCGE). The oxidative DNA damage marker,8,hydroxy,2',deoxyguanosine (8,OHdG), was detected by high performance liquid chromatography with electrochemical detection (HPLC,EC). Results Acrolein could cause DNA strand breakage but not cause DNA,DNA, DNA,protein crosslinks of lymphocytes of human peripheral blood. The reaction of acrolein with DNA in vitro is weak, but the oxidative ability could be enhanced mediated by Fe2+ and the reaction could produce a number of 8,OHdG adducts. The animal experiment showed that acrolein could cause oxidative DNA damage of rat lung tissues with the production of a little 8,OHdG. Conclusion The results of in vitro and in vivo experiments suggests that acrolein had the direct genotoxicity and production of free radicals that induced the oxidative DNA damage. This might be the main way of its genotoxic effects.
Keywords:DNA damage  Acrolein  Genotoxicity  8  hydroxy  2'  deoxyguanosine (8  OHdG)
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号