不同分化喉鳞状细胞癌蛋白质组差异表达的研究

目的 研究不同分化程度喉鳞状细胞癌黏膜组织蛋白质组的差异表达.方法 用固相pH梯度双向凝胶电泳分离不同分化程度喉癌黏膜组织总蛋白质,行考马斯亮蓝染色,Image Master 2D软件分析所获得的双向凝胶电泳图谱,找出差异表达的蛋白质点.结果 获得分辨率和重复性均较好的双向凝胶电泳图谱,发现13种蛋白质与喉癌分化密切相关.其中有7种蛋白质在高分化喉癌组织中表达上调,6种蛋白质表达明显下调.结论 利用固相pH梯度双向凝胶电泳技术分离不同分化喉鳞状细胞癌黏膜组织总蛋白质,建立了重复性较强的不同分化喉鳞状细胞癌黏膜组织蛋白质组表达图谱,且两者蛋白质的表达明显不同,可能与喉癌不同分化程度的发病机制有关.

Comparative proteomic analysis of differently differentiated human laryngeal squamous cell cancer

Objective To establish two-dimensional electrophoresis profiles of laryngeal squamous carcinoma tissue with high resolution and reproducibility. Methods The total protein of well-differentiated or poorly-differentiated laryngeal cancer tissue was separated by means of immobilized pH gradient-based two-dimensional gel electrophoresis (2-DE). The differential expression protein was analyzed by using ImageMaster 2D analysis software. Results We obtained fine resolution, reproducible 2-DE patterns of well-differentiated and poorly-differentiated laryngeal squamous carcinoma. Differential expression protein spots were defined in 2-DE gels. Thirteen protein spots were preliminarily identified, among which seven proteins were upregulated in well-differentiated laryngeal cancer tissue, six were downregulated. Conclusion The expression map of proteomics was established for well-differentiated and poorly-differentiated laryngeal cancer, which was of significant difference between them. Laryngeal cancer proteome might reflect the underlying pathological state.