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Studies on arsenic trioxide induced mice melanoma Cloundman S91 cell apoptosis
引用本文:Ruzhi Zhang,Wenyuan Zhu,Chen Wang. Studies on arsenic trioxide induced mice melanoma Cloundman S91 cell apoptosis[J]. 南京医科大学学报(自然科学版), 2005, 19(3): 139-143
作者姓名:Ruzhi Zhang  Wenyuan Zhu  Chen Wang
作者单位:Ruzhi Zhang~1,Wenyuan Zhu~1 and Chen Wang~2 1Department of Dermatology. the First Affiliated Hospital of Nanjing Medical University. Nanjing 210029,China; 2Department of Dermatology. the Chin-Japa Friendly Hospital. Beijing 100029,China.
摘    要:INTRODUCTION ArsenichasalonghistoryofuseinChineseandwesternmedicine,buthasbeenoutofuseinthe mid20thcenturybecauseoftheunacceptablesideef fects.There emergenceofarsenictrioxideinclinicaluse isduelargelytopurificationofthiscompoundfromtra ditionalmixturesandtheAs2O3definitionofeffective,low doseregimensforthetreatmentofacutepromyelo cyticleukemia(APL)[1].Recently,theapoptosis As2O3inducedhasbeenobservedinmanycancercell lines,includingesophagedcarcinoma,gastriccancer,neuroblastomalymphoid…

关 键 词:三氧化砷 小鼠 动物模型 黑素瘤 S91 细胞凋亡

Studies on arsenic trioxide induced mice melanoma Cloundman S91 cell apoptosis
Ruzhi Zhang,Wenyuan Zhu,Chen Wang. Studies on arsenic trioxide induced mice melanoma Cloundman S91 cell apoptosis[J]. Acta Universitatis Medicinalis Nanjing, 2005, 19(3): 139-143
Authors:Ruzhi Zhang  Wenyuan Zhu  Chen Wang
Affiliation:1. Department of Dermatology. the First Affiliated Hospital of Nanjing Medical University. Nanjing 210029, China
2. Department of Dermatology. the Chin-Japa Friendly Hospital. Beijing 100029, China
Abstract:Objective: To invesfgate the effects of various concentrations As2O3 on malignant melanoma. Methods: The viability of Cloundman melanoma S91 cells treated with As2O3 was measured by MTT [3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide] assay. The apoptosis was determined by transferase-mediated dUTP nick end labeling (TUNEL) detection, and the morphology of apoptotic cells was observed through transmission electron microscopy (TEM). The cells growth phase were analyzed by flow cytometry (FCM). Results: A time-and dose-dependent decrease in cell viability was induced in S91 cells after treatment with As2Os at the concentration of 1-5 μmol/L respectively. The TUNEL indices were 0.033 ± 0.018, 0.062 ± 0.012, 0.102 ± 0.016, 0.132 ± 0.031,and 0.162 ± 0.027 respectively, which were much higher compared with the control group (0.017 ± 0.004, P < 0.01). The flow cytometry showed that hypodiploid peak after treatment with 3 μmol/L and 5 μmol/L of As2O3 for 48 h were 9.99% and 17.59% respectively,which increased significantly compared with the control cells (3.05% P < 0.01, ). The apoptotic morphology observed by transmission electron microscope showed the chromatin became condensed and attached to the inner surface of nuclear membrane. Conclusion: Arsenic trioxide can induce melanoma Cloundman S91 cell apoptosis at the concentration of 1-5 μmol/L, which will provide enhanced benefit in melanoma therapy.
Keywords:arsenic trioxide  melanoma Cloundman S91  apoptosis
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