| HPLC法测定射干根茎与须根中鸢尾苷的含量 |
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| 引用本文： | 姜晓峰. HPLC法测定射干根茎与须根中鸢尾苷的含量[J]. 海峡药学, 2014, 0(5): 31-32 |
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| 作者姓名： | 姜晓峰 |
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| 作者单位： | 浙江温州市中医院,温州325000 |
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| 摘 要： | 目的：采用HPLC法测定射干根茎与须根中鸢尾苷的含量，并比较两者含量。方法采用 Eclipse XDB-C18色谱柱（250mm×4.6mm，5μm），以乙腈-0.2％磷酸溶液（20∶80）为流动相，流速1.0mL·min -1，检测波长266nm，进样量20μL，柱温35℃。结果鸢尾苷进样量在3.1875～47μg范围内与峰面积线性关系良好，r＝0.9998，精密度、重复性、稳定性良好，平均加样回收率为99.81％。结论所建方法可同时测定射干根茎与须根中鸢尾苷的含量，并且根茎中的鸢尾苷含量显著高于须根。
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| 关 键 词： | 射干 鸢尾苷 高效液相色谱法 |
Determination of Tectoridin in rhizome and beard of Belamcanda Chinen-sis by HPLC |
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| JIANG Xiao-feng. Determination of Tectoridin in rhizome and beard of Belamcanda Chinen-sis by HPLC[J]. Strait Pharmaceutical Journal, 2014, 0(5): 31-32 |
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| Authors: | JIANG Xiao-feng |
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| Affiliation: | JIANG Xiao-feng (Traditional Chinese Medicine Hospital of Zhejiang Province Wenzhou city, Wenzhou 325000, China) |
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| Abstract: | OBJECTIVE To establish a HPLC method for determining the content of tectoridin in Belamcanda Chinensis and to compare the content of tectoridin in rhizome and beard.METHODS The assay was performed on a Eclipse XDB-C18 column （250mm ×4.6mm,5μm）;eluted with a mobile phase of acetonitrile-0.2%phosphoric acid （20∶80） at a flow rate of 1.0mL·min-1;The detection wavelength was 266nm and the column temperature was 35℃.Results The linear range of irisflorentin was 3.1875 -47μg （ r =0.9998 ）.The reproducibility and stability were good and the average recovery was 99.81%.CONCLUSION The method is simple、rapid and reliable.The content of tectoridin in rhizome is by far higher than that in beard. |
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| Keywords: | Belamcanda Chinensis Tectoridin HPLC |
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