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| 微囊化牛嗜铬细胞移植对致痛大鼠行为学及脊髓C-fos表达的影响 | |
| 引用本文： | 游言文,赵文峰,臧卫东,李鸣.微囊化牛嗜铬细胞移植对致痛大鼠行为学及脊髓C-fos表达的影响[J].郑州大学学报(医学版),2005,40(3):436-438. |
| 作者姓名： | 游言文 赵文峰 臧卫东 李鸣 |
| 作者单位： | 1. 河南中医学院解剖与组胚学科,郑州,450008 2. 河南中医学院数理学科,郑州,450008 3. 郑州大学基础医学院人体解剖学教研室,郑州,450052 |
| 基金项目： | 河南省科技攻关基金资助项目0224630010 |
| 摘 要： | 目的:探讨微囊化牛嗜铬细胞(BCC)蛛网膜下腔移植对疼痛大鼠脊髓后角神经元活动的影响。方法:SD大鼠24只,随机分为空囊组、微囊化BCC组和BCC组,每组8只。分别在3组大鼠的脊髓蛛网膜下腔植入含空囊的DMEM、微囊化BCC和单纯BCC。移植8周后,对3组大鼠行福尔马林疼痛实验;福尔马林处理后2h,免疫组织化学法检测大鼠脊髓后角Cfos蛋白的表达。结果:空囊组、BCC组和微囊化BCC组大鼠痛觉行为反应和脊髓后角Cfos阳性神经元数目均依次减少(P均<0.05)。结论:BCC移植对福尔马林致痛大鼠有镇痛作用,可以抑制福尔马林致痛大鼠脊髓后角Cfos的表达,微囊化BCC效果优于BCC。 |
| 关 键 词： | APA微囊 嗜铬细胞 疼痛 镇痛 C-fos |
| 修稿时间： | 2004年12月22 |
Effect of transplantation of microencapsulated bovine chromaffin cells on behavior and C-fos expression in spinal cord of formalin-induced rats |
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| You Yanwen,Zhao Wenfeng,ZANG Weidong,LI Ming.Effect of transplantation of microencapsulated bovine chromaffin cells on behavior and C-fos expression in spinal cord of formalin-induced rats[J].Journal of Zhengzhou University: Med Sci,2005,40(3):436-438. | |
| Authors: | You Yanwen Zhao Wenfeng ZANG Weidong LI Ming |
| Institution: | YOU Yanwen 1),ZHAO Wenfeng 2),ZANG Weidong 3),LI Ming 3) 1)Department of Anatomy and Embryology,Henan College of Traditional Chinese Medicine,Zhengzhou 450008 2)Department of Mathemetics,Henan College of Traditional Chinese Medicine,Zhengzhou 450008 3)Department of Human Anatomy,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450052 |
| Abstract: | Aim: To observe the effect of transplantation of microencapsulated bovine chromaffin cells(BCCs) on activity of nerve cells in spinal dorsal cord of formalin-induced rats.Methods: A total of 24 SD rats were randomly allocated into APA group, APA-BCC group and BCC group.DMEM including empty microcapsules,APA microencapsulated BCC,BCCs were injected into the spinal subarachnoid space of rats in the three groups,respectively. Eight weeks after the operations, the formalin test was performed on the rats in three groups.At 2 h after injection of formalin, the expression of C-fos protein in spinal dorsal cord was detected using immunohistochemical method.Results: The reflects to formalin and the number of C-fos-positive nerve cells in the spinal cord of rats in APA gronp, APA-BCC group and BCC group,decreased consequently(P<0.05). Conclusion: Transplantation of microencapsulated BCC can produce analgesic effect on formalin-induced rats, and reduce the expression of C-fos in spinal dorsal cord. |
| Keywords: | APA microcapsule chromaffin cell pain analgesia C-fos |
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