新型植骨材料-复合rhBMP_2的异种骨的实验研究

目的探讨复合ｒｈＢＭＰ２的异种骨（ｅｈＢＭＰ２／ＢＣＢ）移植修复节段性骨缺损的效果。方法在重组合异种骨（ＲＢＸ）的基础上，以基因重组人ＢＭＰ２（ｒｈＢＭＰ２）与去抗原牛松质骨载体（ＢＣＢ）复合，制成一种新型植骨材料复合ｒｈＢＭＰ２的异种骨（ｒｈＢＭＰ２／ＢＣＢ），并采用兔桡骨干１．５ｃｍ缺损动物模型，通过Ｘ线、骨密度、生物力学、组织学等检测手段，对ｒｈＢＭＰ２／ＢＣＢ移植治疗节段性骨缺损效果进行研究。结果单纯ｒｈＢＭＰ２／ＢＣＢ移植可在１６周使骨缺损基本修复，其机制和过程与重组合异种骨相似。结论ｒｈＢＭＰ２／ＢＣＢ是一种新型理想的修复节段性骨缺损的移植材料。

rhBMP2/BCB repair segmental defects in radii of rabbits

Objective To study the efficacy of rhBMP2/BCB in repairing segmental bony defects. Method After successful application in clinical settings of the reconstituted bone xenograft(RBX), A new grafting materiala xenograft containing rhBMP2 (rhBMP2 / B C B ) was made by combining recombinant - human BMP2 (rhBMP2) with an antigen - ftee bovine cancellous bone (BCB). The new product was used in repairing a 1. 5cm defect in the radius of the rabbit. Its defect - repairing capacity was assessed radiographically and biomechenicall by densitometry and histological studies. Result rhBMP2/BCB was capable of healing the defect in large part by 16 weeks, with a simiar repair process and mechanism to that seen with RBX. Conclusion rh BMP2/BCB is believed to be a new preferred grafting material in dealing with segmental bony defects.