| The effects of soybean isoflavaones on the mRNA expression of OPG, ODF and M-CSF in bone tissue of ovariectomized rat |
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| 作者姓名： | Qiang Wang Kunzheng Wang Xiaoqian Dang |
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| 作者单位： | Department of Orthopaedics Surgery, the Second Hospital of Xi＇an Jiaotong University, Xi＇an 710004, China |
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| 摘 要： | Objective： To observe the effects of the soybean isoflavaones on the rnRNA expression of osteoprotegerin（OPG）, osteoclast differentiation factor（ODF） and macrophage colony stimulating factor（M-CSF） in bone tissue of ovariectomized rat, and to investigate the possible pathway to prevent and treat postrmenopausal osteoporosis using soybean isoflavaones. Methods： Thirty healthy adult SD rats were randomly divided into 3 groups： sham-operated group, ovariectomized group and soybean isoflavaones treated group. All rats were ovariectomized except those in sham-operated group. The bone density of the 3-6th lumbar vertebrae was detected after 12 weeks. Total RNA was extracted from femur bone and the mRNA expression of OPG, ODF and M-CSF was examined by real time PCR. Results： Soybean isoflavaones increased the bone density of the lumbar vertebrae in ovariectomized rat and up-regulated the expression of OPG, whereas down-regulated the expression of M-CSF and the ratio of ODF to OPG. Conclusion： The effects of soybean isoflavaones on postmenopausal osteoporosis are tightly correlated with OPG, M-CSF and the ratio of ODF to OPG.
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| 收稿时间： | 2005-06-20 |
The effects of soybean isoflavaones on the mRNA expression of OPG,ODF and M-CSF in bone tissue of ovariectomized rat |
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| Qiang Wang,Kunzheng Wang,Xiaoqian Dang.The effects of soybean isoflavaones on the mRNA expression of OPG, ODF and M-CSF in bone tissue of ovariectomized rat[J].Acta Universitatis Medicinalis Nanjing,2006,20(2):101-103. |
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| Authors: | Qiang Wang Kunzheng Wang Xiaoqian Dang |
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| Abstract: | Objective: To observe the effects of the soybean isoflavaones on the mRNA expression of osteoprotegerin(OPG), osteoclast differentiation factor(ODF) and macrophage colony stimulating factor(M-CSF) in bone tissue of ovariectomized rat, and to investigate the possible pathway to prevent and treat postmenopausal osteoporosis using soybean isoflavaones. Methods: Thirty healthy adult SD rats were randomly divided into 3 groups: sham-operated group, ovariectomized group and soybean isoflavaones treated group. All rats were ovariectomized except those in sham-operated group. The bone density of the 3-6th lumbar vertebrae was detected after 12 weeks. Total RNA was extracted from femur bone and the mRNA expression of OPG, ODF and M-CSF was examined by real time PCR. Results: Soybean isoflavaones increased the bone density of the lumbar vertebrae in ovariectomized rat and up-regulated the expression of OPG, whereas down-regulated the expression of M-CSF and the ratio of ODF to OPG. Conclusion: The effects of soybean isoflavaones on postmenopausal osteoporosis are tightly correlated with OPG, M-CSF and the ratio of ODF to OPG. |
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| Keywords: | soybean isoflavaones osteoporosis cytokine bone density |
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